TY - JOUR

T1 - Mathematical Modeling of Continuous Separation Process of Proteins using Partial Recycling Free-Flow Isoelectric Focusing with Feed Supply and Product Withdrawal

AU - Kim, Seong Hoon

AU - Otani, Ritsuko

AU - Shira, Wataru

AU - Shibasaki-Kitakawa, Naomi

AU - Yonemoto, Toshikuni

AU - Kitakawa, Akio

AU - Otani, Ritsuko

AU - Shira, Wataru

PY - 2001

Y1 - 2001

N2 - An unsteady state mathematical model describing the continuous separation process of a binary mixture of protein using partial recycling free-flow isoelectric focusing is constructed. The model consists of a one-dimensional mass balance equation and an electric charge balance equation for the electrophoretic chamber and a mass balance equation for the reservoir. The model equations were solved numerically using a finite difference method with literature values of physical constants such as dissociation and diffusion constants. The calculated results are in good agreement with the experimental data for the separation of protein mixture of bovine serum albumin (BSA) and bovine hemoglobin (Hb) reported previously. Furthermore, the model can describe the effects of the operating factors, such as support electrolyte concentration and feed flow rate, on the separation process. Key words: bioseparation, isoelectric focusing, protein, bovine serum albumin, bovine hemoglobin, mathematical modeling.

AB - An unsteady state mathematical model describing the continuous separation process of a binary mixture of protein using partial recycling free-flow isoelectric focusing is constructed. The model consists of a one-dimensional mass balance equation and an electric charge balance equation for the electrophoretic chamber and a mass balance equation for the reservoir. The model equations were solved numerically using a finite difference method with literature values of physical constants such as dissociation and diffusion constants. The calculated results are in good agreement with the experimental data for the separation of protein mixture of bovine serum albumin (BSA) and bovine hemoglobin (Hb) reported previously. Furthermore, the model can describe the effects of the operating factors, such as support electrolyte concentration and feed flow rate, on the separation process. Key words: bioseparation, isoelectric focusing, protein, bovine serum albumin, bovine hemoglobin, mathematical modeling.

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U2 - 10.1252/kakoronbunshu.27.174

DO - 10.1252/kakoronbunshu.27.174

M3 - Article

AN - SCOPUS:23044525469

SN - 0386-216X

VL - 27

SP - 174

EP - 180

JO - Kagaku Kogaku Ronbunshu

JF - Kagaku Kogaku Ronbunshu

IS - 2

ER -