MC3T3-E1 cell response to hydroxyapatite and alpha-type alumina adsorbed with bovine serum albumin

Jumpei Hayashi, Masakazu Kawashita, Toshiki Miyazaki, Tada Aki Kudo, Hiroyasu Kanetaka, Masami Hashimoto

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

MC3T3-E1 cell responses, such as cell adhesion and proliferation, to original and bovine serum albumin (BSA) coated disc (original-disc, BSA-disc) of hydroxyapatite (HA) or alpha-type alumina (α-Al2O3) was studied. There was no significant difference in the cell proliferation between BSA-discs and original-discs even after incubated for 14 days, but the cell number at day 14 tended to be higher (p = 0.054) on the BSA-discs of HA than on the original-discs of HA. Incidentally, the amount of adsorbed protein was higher on BSA-discs than on original-discs only until incubated in culture medium for 3 h. BSA adsorption might influence the MC3T3-E1 cell adhesion to HA, as a result the specific adsorption of albumin on HA is likely to affect the expression of the osteoconductivity of materials.

Original languageEnglish
Title of host publicationBioceramics 24
PublisherTrans Tech Publications Ltd
Pages365-369
Number of pages5
Edition1
ISBN (Print)9783037855171
DOIs
Publication statusPublished - 2013
Event24th Symposium and Annual Meeting of International Society for Ceramics in Medicine, ISCM 2012 - Fukuoka, Japan
Duration: 2012 Oct 212012 Oct 24

Publication series

NameKey Engineering Materials
Number1
Volume529-530
ISSN (Print)1013-9826
ISSN (Electronic)1662-9795

Conference

Conference24th Symposium and Annual Meeting of International Society for Ceramics in Medicine, ISCM 2012
Country/TerritoryJapan
CityFukuoka
Period12/10/2112/10/24

Keywords

  • Albumin
  • Alumina
  • Hydroxyapatite
  • Osteoblast
  • Osteoconductivity

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