Measurement of abscisic acid and gibberellins by gas chromatography/mass spectrometry

Masanori Okamoto, Atsushi Hanada, Yuji Kamiya, Shinjiro Yamaguchi, Eiji Nambara

Research output: Chapter in Book/Report/Conference proceedingChapter

20 Citations (Scopus)


Gas chromatography-mass spectrometry (GC-MS)-based analysis is an accurate and sensitive method to quantify plant hormones. This method is commonly used for analysis of low-molecular-weight compounds, such as abscisic acid (ABA), gibberellins (GAs), auxins, and brassinosteroids. Procedures are composed of four major steps: extraction, fractionation, derivatization, and detection. Here, we describe a protocol for quantification of ABA and GAs.

Original languageEnglish
Title of host publicationPlant Hormones
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
Number of pages8
ISBN (Print)9781934115329
Publication statusPublished - 2009
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Abscisic acid
  • Derivatization
  • Gas chromatography-mass spectrometry (GC-MS)
  • Gibberellins
  • Internal standard

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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