Measurement of abscisic acid and gibberellins by gas chromatography/mass spectrometry

Masanori Okamoto; Atsushi Hanada; Yuji Kamiya; Shinjiro Yamaguchi; Eiji Nambara

doi:10.1007/978-1-59745-477-3_5

Measurement of abscisic acid and gibberellins by gas chromatography/mass spectrometry

Masanori Okamoto, Atsushi Hanada, Yuji Kamiya, Shinjiro Yamaguchi, Eiji Nambara

Research output: Chapter in Book/Report/Conference proceeding › Chapter

20 Citations (Scopus)

Abstract

Gas chromatography-mass spectrometry (GC-MS)-based analysis is an accurate and sensitive method to quantify plant hormones. This method is commonly used for analysis of low-molecular-weight compounds, such as abscisic acid (ABA), gibberellins (GAs), auxins, and brassinosteroids. Procedures are composed of four major steps: extraction, fractionation, derivatization, and detection. Here, we describe a protocol for quantification of ABA and GAs.

Original language	English
Title of host publication	Plant Hormones
Subtitle of host publication	Methods and Protocols
Publisher	Humana Press
Pages	53-60
Number of pages	8
ISBN (Print)	9781934115329
DOIs	https://doi.org/10.1007/978-1-59745-477-3_5
Publication status	Published - 2009
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	495
ISSN (Print)	1064-3745

Keywords

Abscisic acid
Derivatization
Gas chromatography-mass spectrometry (GC-MS)
Gibberellins
Internal standard

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-59745-477-3_5

Cite this

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T1 - Measurement of abscisic acid and gibberellins by gas chromatography/mass spectrometry

AU - Okamoto, Masanori

AU - Hanada, Atsushi

AU - Kamiya, Yuji

AU - Yamaguchi, Shinjiro

AU - Nambara, Eiji

PY - 2009

Y1 - 2009

N2 - Gas chromatography-mass spectrometry (GC-MS)-based analysis is an accurate and sensitive method to quantify plant hormones. This method is commonly used for analysis of low-molecular-weight compounds, such as abscisic acid (ABA), gibberellins (GAs), auxins, and brassinosteroids. Procedures are composed of four major steps: extraction, fractionation, derivatization, and detection. Here, we describe a protocol for quantification of ABA and GAs.

AB - Gas chromatography-mass spectrometry (GC-MS)-based analysis is an accurate and sensitive method to quantify plant hormones. This method is commonly used for analysis of low-molecular-weight compounds, such as abscisic acid (ABA), gibberellins (GAs), auxins, and brassinosteroids. Procedures are composed of four major steps: extraction, fractionation, derivatization, and detection. Here, we describe a protocol for quantification of ABA and GAs.

KW - Abscisic acid

KW - Derivatization

KW - Gas chromatography-mass spectrometry (GC-MS)

KW - Gibberellins

KW - Internal standard

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ER -

Measurement of abscisic acid and gibberellins by gas chromatography/mass spectrometry

Abstract

Publication series

Keywords

ASJC Scopus subject areas

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