TY - JOUR
T1 - Measurement of phosphatidylcholine hydroperoxide in mild ischemia-reperfusion injury in rat intestine
AU - Masuko, Tsuyoshi
AU - Funayama, Yuji
AU - Naito, Hiroo
AU - Fukushima, Kohei
AU - Shibata, Chikashi
AU - Takahashi, Ken Ichi
AU - Ogawa, Hitoshi
AU - Ueno, Tatsuya
AU - Hashimoto, Akihiko
AU - Miyazawa, Teruo
AU - Nakagawa, Kiyotaka
AU - Matsuno, Seiki
AU - Sasaki, Iwao
PY - 2002/12/1
Y1 - 2002/12/1
N2 - In the pathogenesis of intestinal ischemia-reperfusion injury, the measurement of lipid peroxides needs to be established. Sprague-Dawley rat intestines were assessed after 30 minutes of occlusion of the superior mesenteric artery followed by reperfusion at 30, 60, 120, 180, 360 minutes. Grade of the mucosal injury, accumulation of the activated neutrophils and ICAM-1 expression were transiently increased after reperfusion. Two measuring methods of mucosal lipid peroxides using thiobarbituric acid reacting substance (TBARS) and phosphatidylcholine hydroperoxide (PCOOH) were compared. PCOOH level was significantly increased after reperfusion, while the mucosal TBARS level showed no significant change. In conclusion, lipid peroxidation could be detected with high specificity and sensitivity by measuring the mucosal phosphatidylcholine hydroperoxide level.
AB - In the pathogenesis of intestinal ischemia-reperfusion injury, the measurement of lipid peroxides needs to be established. Sprague-Dawley rat intestines were assessed after 30 minutes of occlusion of the superior mesenteric artery followed by reperfusion at 30, 60, 120, 180, 360 minutes. Grade of the mucosal injury, accumulation of the activated neutrophils and ICAM-1 expression were transiently increased after reperfusion. Two measuring methods of mucosal lipid peroxides using thiobarbituric acid reacting substance (TBARS) and phosphatidylcholine hydroperoxide (PCOOH) were compared. PCOOH level was significantly increased after reperfusion, while the mucosal TBARS level showed no significant change. In conclusion, lipid peroxidation could be detected with high specificity and sensitivity by measuring the mucosal phosphatidylcholine hydroperoxide level.
KW - Intestine
KW - Ischemia-reperfusion injury
KW - Lipid peroxide
KW - Rat
UR - http://www.scopus.com/inward/record.url?scp=0036928386&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036928386&partnerID=8YFLogxK
U2 - 10.1620/tjem.198.223
DO - 10.1620/tjem.198.223
M3 - Article
C2 - 12630554
AN - SCOPUS:0036928386
SN - 0040-8727
VL - 198
SP - 223
EP - 232
JO - Tohoku Journal of Experimental Medicine
JF - Tohoku Journal of Experimental Medicine
IS - 4
ER -