TY - JOUR
T1 - Measurement of Srs-A Activities in Plasma of Asthmatic Patients
AU - Ohtsu, Hiroshi
AU - Mue, Suetsugu
AU - Tamura, Gen
AU - Yamauchi, Kohei
AU - Ishihara, Toshiharu
AU - Sato, Kohichi
AU - Takishima, Tamotsu
PY - 1985
Y1 - 1985
N2 - Ethanol extracts of arterial blood from 20 of 29 asthmatic patients during attacks produced sustained contractions of the guinea pig ileum. These contractions were inhibited by FPL 55712, a specific antagonist of slow reacting substance of anaphylaxis (SRS-A). SRS-A activity was significantly higher during attack than during remission. Reverse-phase high pressure liquid chromatography (HPLC) was performed on two extracts which had shown high SRS-A activity with bioassay. One extract showed double peaks, which had retention times identical to those of leukotriene (LT) C4 and LTD4. The other extract showed a single peak, which had a retention time identical to that of synthetic LTC4. bronchial asthma; slow reacting substance of anaphylaxis (SRS-A) ; bioassay ; high pressure liquid chromatography (HPLC).
AB - Ethanol extracts of arterial blood from 20 of 29 asthmatic patients during attacks produced sustained contractions of the guinea pig ileum. These contractions were inhibited by FPL 55712, a specific antagonist of slow reacting substance of anaphylaxis (SRS-A). SRS-A activity was significantly higher during attack than during remission. Reverse-phase high pressure liquid chromatography (HPLC) was performed on two extracts which had shown high SRS-A activity with bioassay. One extract showed double peaks, which had retention times identical to those of leukotriene (LT) C4 and LTD4. The other extract showed a single peak, which had a retention time identical to that of synthetic LTC4. bronchial asthma; slow reacting substance of anaphylaxis (SRS-A) ; bioassay ; high pressure liquid chromatography (HPLC).
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U2 - 10.1620/tjem.145.197
DO - 10.1620/tjem.145.197
M3 - Article
C2 - 3992584
AN - SCOPUS:0021987930
SN - 0040-8727
VL - 145
SP - 197
EP - 203
JO - Tohoku Journal of Experimental Medicine
JF - Tohoku Journal of Experimental Medicine
IS - 2
ER -