Melanin-concentrating hormone in the human brain

Toraichi Mouri; Kazuhiro Takahashi; Hiroshi Kawauchi; Masahiko Sone; Kazuhito Totsune; Osamu Murakami; Keiichi Itoi; Makoto Ohneda; Hironobu Sasano; Nobuaki Sasano

doi:10.1016/0196-9781(93)90158-D

Melanin-concentrating hormone in the human brain

Toraichi Mouri, Kazuhiro Takahashi, Hiroshi Kawauchi, Masahiko Sone, Kazuhito Totsune, Osamu Murakami, Keiichi Itoi, Makoto Ohneda, Hironobu Sasano, Nobuaki Sasano

Research output: Contribution to journal › Article › peer-review

57 Citations (Scopus)

Abstract

The presence of human melanin-concentrating hormone (MCH) was studied in the human brain by radioimmunoassay and immunocytochemistry. Immunoreactive MCH concentrations in the human brain ranged from 0.07 to 19.7 pmol/g wet weight. High performance liquid chromatography of the hypothalamus showed a large immunoreactive peak in the position of human/rat MCH, which was eluted 9 min later than that of salmon MCH. Free-floating sections (40 μm) of the hypothalamus were immunostained. Positive MCH immunostaining was found in perifornical, tuberomammillary, and posterior nuclei. Numerous MCH-immunoreactive nerve fibers were observed throughout the hypothalamus. The presence of high concentrations of MCH in the human brain, in particular in the hypothalamus, suggests that MCH is a neurotransmitter, a neuromodulator, or a neurohormone in man.

Original language	English
Pages (from-to)	643-646
Number of pages	4
Journal	Peptides
Volume	14
Issue number	3
DOIs	https://doi.org/10.1016/0196-9781(93)90158-D
Publication status	Published - 1993

Keywords

Brain
Chromatography
Human
Hypothalamus
Immunocytochemistry
Melanin-concentrating hormone
Radioimmunoassay

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

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10.1016/0196-9781(93)90158-D

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title = "Melanin-concentrating hormone in the human brain",

abstract = "The presence of human melanin-concentrating hormone (MCH) was studied in the human brain by radioimmunoassay and immunocytochemistry. Immunoreactive MCH concentrations in the human brain ranged from 0.07 to 19.7 pmol/g wet weight. High performance liquid chromatography of the hypothalamus showed a large immunoreactive peak in the position of human/rat MCH, which was eluted 9 min later than that of salmon MCH. Free-floating sections (40 μm) of the hypothalamus were immunostained. Positive MCH immunostaining was found in perifornical, tuberomammillary, and posterior nuclei. Numerous MCH-immunoreactive nerve fibers were observed throughout the hypothalamus. The presence of high concentrations of MCH in the human brain, in particular in the hypothalamus, suggests that MCH is a neurotransmitter, a neuromodulator, or a neurohormone in man.",

keywords = "Brain, Chromatography, Human, Hypothalamus, Immunocytochemistry, Melanin-concentrating hormone, Radioimmunoassay",

author = "Toraichi Mouri and Kazuhiro Takahashi and Hiroshi Kawauchi and Masahiko Sone and Kazuhito Totsune and Osamu Murakami and Keiichi Itoi and Makoto Ohneda and Hironobu Sasano and Nobuaki Sasano",

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doi = "10.1016/0196-9781(93)90158-D",

language = "English",

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AU - Mouri, Toraichi

AU - Takahashi, Kazuhiro

AU - Kawauchi, Hiroshi

AU - Sone, Masahiko

AU - Totsune, Kazuhito

AU - Murakami, Osamu

AU - Itoi, Keiichi

AU - Ohneda, Makoto

AU - Sasano, Hironobu

AU - Sasano, Nobuaki

PY - 1993

Y1 - 1993

N2 - The presence of human melanin-concentrating hormone (MCH) was studied in the human brain by radioimmunoassay and immunocytochemistry. Immunoreactive MCH concentrations in the human brain ranged from 0.07 to 19.7 pmol/g wet weight. High performance liquid chromatography of the hypothalamus showed a large immunoreactive peak in the position of human/rat MCH, which was eluted 9 min later than that of salmon MCH. Free-floating sections (40 μm) of the hypothalamus were immunostained. Positive MCH immunostaining was found in perifornical, tuberomammillary, and posterior nuclei. Numerous MCH-immunoreactive nerve fibers were observed throughout the hypothalamus. The presence of high concentrations of MCH in the human brain, in particular in the hypothalamus, suggests that MCH is a neurotransmitter, a neuromodulator, or a neurohormone in man.

AB - The presence of human melanin-concentrating hormone (MCH) was studied in the human brain by radioimmunoassay and immunocytochemistry. Immunoreactive MCH concentrations in the human brain ranged from 0.07 to 19.7 pmol/g wet weight. High performance liquid chromatography of the hypothalamus showed a large immunoreactive peak in the position of human/rat MCH, which was eluted 9 min later than that of salmon MCH. Free-floating sections (40 μm) of the hypothalamus were immunostained. Positive MCH immunostaining was found in perifornical, tuberomammillary, and posterior nuclei. Numerous MCH-immunoreactive nerve fibers were observed throughout the hypothalamus. The presence of high concentrations of MCH in the human brain, in particular in the hypothalamus, suggests that MCH is a neurotransmitter, a neuromodulator, or a neurohormone in man.

KW - Brain

KW - Chromatography

KW - Human

KW - Hypothalamus

KW - Immunocytochemistry

KW - Melanin-concentrating hormone

KW - Radioimmunoassay

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AN - SCOPUS:0027298381

SN - 0196-9781

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JO - Peptides

JF - Peptides

IS - 3

ER -

Melanin-concentrating hormone in the human brain

Abstract

Keywords

ASJC Scopus subject areas

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