Method for Quantitative Determination of Cerebroside in “Plant Ceramide” Foodstuffs by High Performance Liquid Chromatography with Evaporative Light Scattering Detection

Minoru Kashima; Kiyotaka Nakagawa; Tatsuya Sugawara; Teruo Miyazawa; Chiaki Murakami; Rumiko Miyashita; Jisaburo Ono; F. S. Deschamps; P. Chaminade

doi:10.5650/jos.51.347

Method for Quantitative Determination of Cerebroside in “Plant Ceramide” Foodstuffs by High Performance Liquid Chromatography with Evaporative Light Scattering Detection

Minoru Kashima, Kiyotaka Nakagawa, Tatsuya Sugawara, Teruo Miyazawa, Chiaki Murakami, Rumiko Miyashita, Jisaburo Ono, F. S. Deschamps, P. Chaminade

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Normal-phase high performance liquid chromatography was developed with evaporative light scattering detection (HPLC-ELSD) for determining cerebroside (monoglycosylceramide) present in commercially available “Plant ceramide”. Authentic cerebroside, isolated from wheat flour by preparative HPLC, served as the standard. A standard curve was obtained for what at 1 to 40 μg of cerebroside, the equation for which was y=axb (x: cerebroside weight; y: peak area). The regression correlation coefficients was above 0.997. Prior to making determination, food samples were saponified so as to remove glycerolipids, and thus improve the precision of cerebroside. Determination on three foodstuffs each containing cerebroside, the present method was conducted and evaluated has on the resulted. Statistically significant intra- and inter-laboratory variation in the data were analyzed. The present HPLC-ELSD was shown quite effective for accurately determining cerebroside content in “Plant ceramide” food samples.

Original language	English
Pages (from-to)	347-354
Number of pages	8
Journal	Journal of Oleo Science
Volume	51
Issue number	5
DOIs	https://doi.org/10.5650/jos.51.347
Publication status	Published - 2002

Keywords

cerebroside
evaporative light scattering detection

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10.5650/jos.51.347

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Kashima, M., Nakagawa, K., Sugawara, T., Miyazawa, T., Murakami, C., Miyashita, R., Ono, J., Deschamps, F. S., & Chaminade, P. (2002). Method for Quantitative Determination of Cerebroside in “Plant Ceramide” Foodstuffs by High Performance Liquid Chromatography with Evaporative Light Scattering Detection. Journal of Oleo Science, 51(5), 347-354. https://doi.org/10.5650/jos.51.347

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title = "Method for Quantitative Determination of Cerebroside in “Plant Ceramide” Foodstuffs by High Performance Liquid Chromatography with Evaporative Light Scattering Detection",

abstract = "Normal-phase high performance liquid chromatography was developed with evaporative light scattering detection (HPLC-ELSD) for determining cerebroside (monoglycosylceramide) present in commercially available “Plant ceramide”. Authentic cerebroside, isolated from wheat flour by preparative HPLC, served as the standard. A standard curve was obtained for what at 1 to 40 μg of cerebroside, the equation for which was y=axb (x: cerebroside weight; y: peak area). The regression correlation coefficients was above 0.997. Prior to making determination, food samples were saponified so as to remove glycerolipids, and thus improve the precision of cerebroside. Determination on three foodstuffs each containing cerebroside, the present method was conducted and evaluated has on the resulted. Statistically significant intra- and inter-laboratory variation in the data were analyzed. The present HPLC-ELSD was shown quite effective for accurately determining cerebroside content in “Plant ceramide” food samples.",

keywords = "cerebroside, evaporative light scattering detection",

author = "Minoru Kashima and Kiyotaka Nakagawa and Tatsuya Sugawara and Teruo Miyazawa and Chiaki Murakami and Rumiko Miyashita and Jisaburo Ono and Deschamps, {F. S.} and P. Chaminade",

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Kashima, M, Nakagawa, K, Sugawara, T, Miyazawa, T, Murakami, C, Miyashita, R, Ono, J, Deschamps, FS & Chaminade, P 2002, 'Method for Quantitative Determination of Cerebroside in “Plant Ceramide” Foodstuffs by High Performance Liquid Chromatography with Evaporative Light Scattering Detection', Journal of Oleo Science, vol. 51, no. 5, pp. 347-354. https://doi.org/10.5650/jos.51.347

TY - JOUR

T1 - Method for Quantitative Determination of Cerebroside in “Plant Ceramide” Foodstuffs by High Performance Liquid Chromatography with Evaporative Light Scattering Detection

AU - Kashima, Minoru

AU - Nakagawa, Kiyotaka

AU - Sugawara, Tatsuya

AU - Miyazawa, Teruo

AU - Murakami, Chiaki

AU - Miyashita, Rumiko

AU - Ono, Jisaburo

AU - Deschamps, F. S.

AU - Chaminade, P.

PY - 2002

Y1 - 2002

N2 - Normal-phase high performance liquid chromatography was developed with evaporative light scattering detection (HPLC-ELSD) for determining cerebroside (monoglycosylceramide) present in commercially available “Plant ceramide”. Authentic cerebroside, isolated from wheat flour by preparative HPLC, served as the standard. A standard curve was obtained for what at 1 to 40 μg of cerebroside, the equation for which was y=axb (x: cerebroside weight; y: peak area). The regression correlation coefficients was above 0.997. Prior to making determination, food samples were saponified so as to remove glycerolipids, and thus improve the precision of cerebroside. Determination on three foodstuffs each containing cerebroside, the present method was conducted and evaluated has on the resulted. Statistically significant intra- and inter-laboratory variation in the data were analyzed. The present HPLC-ELSD was shown quite effective for accurately determining cerebroside content in “Plant ceramide” food samples.

AB - Normal-phase high performance liquid chromatography was developed with evaporative light scattering detection (HPLC-ELSD) for determining cerebroside (monoglycosylceramide) present in commercially available “Plant ceramide”. Authentic cerebroside, isolated from wheat flour by preparative HPLC, served as the standard. A standard curve was obtained for what at 1 to 40 μg of cerebroside, the equation for which was y=axb (x: cerebroside weight; y: peak area). The regression correlation coefficients was above 0.997. Prior to making determination, food samples were saponified so as to remove glycerolipids, and thus improve the precision of cerebroside. Determination on three foodstuffs each containing cerebroside, the present method was conducted and evaluated has on the resulted. Statistically significant intra- and inter-laboratory variation in the data were analyzed. The present HPLC-ELSD was shown quite effective for accurately determining cerebroside content in “Plant ceramide” food samples.

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Method for Quantitative Determination of Cerebroside in “Plant Ceramide” Foodstuffs by High Performance Liquid Chromatography with Evaporative Light Scattering Detection

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