Normal-phase high performance liquid chromatography was developed with evaporative light scattering detection (HPLC-ELSD) for determining cerebroside (monoglycosylceramide) present in commercially available “Plant ceramide”. Authentic cerebroside, isolated from wheat flour by preparative HPLC, served as the standard. A standard curve was obtained for what at 1 to 40 μg of cerebroside, the equation for which was y=axb (x: cerebroside weight; y: peak area). The regression correlation coefficients was above 0.997. Prior to making determination, food samples were saponified so as to remove glycerolipids, and thus improve the precision of cerebroside. Determination on three foodstuffs each containing cerebroside, the present method was conducted and evaluated has on the resulted. Statistically significant intra- and inter-laboratory variation in the data were analyzed. The present HPLC-ELSD was shown quite effective for accurately determining cerebroside content in “Plant ceramide” food samples.
- evaporative light scattering detection