Micro-second time-resolved X-ray single-molecule internal motions of SARS-CoV-2 spike variants

Daisuke Sasaki, Tatsuya Arai, Yue Yang, Masahiro Kuramochi, Wakako Furuyama, Asuka Nanbo, Hiroshi Sekiguchi, Nobuhiro Morone, Kazuhiro Mio, Yuji C. Sasaki

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Single-molecule intramolecular dynamics were successfully measured for three variants of SARS-CoV-2 spike protein, alpha: B.1.1.7, delta: B.1.617, and omicron: B.1.1.529, with a time resolution of 100 μs using X-rays. The results were then compared with respect to the magnitude and directions of motions for the three variants. The largest 3-D intramolecular movement was observed for the omicron variant irrespective of ACE2 receptor binding. A more detailed analysis of the intramolecular motions revealed that the distribution state of intramolecular motion for the three variants was completely different with and without ACE2 receptor binding. The molecular dynamics for the trimeric spike protein of the omicron variant increased when ACE2 binding occurred. At that time, the diffusion constant increased from 71.0 [mrad2/ms] to 91.1 [mrad2/ms].

Original languageEnglish
Article number101712
JournalBiochemistry and Biophysics Reports
Volume38
DOIs
Publication statusPublished - 2024 Jul

Keywords

  • Diffracted X-ray tracking
  • Micro-second time-resolved measurement
  • SARS-CoV-2 spike protein
  • Single-molecule internal dynamics

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