TY - JOUR
T1 - Micro-second time-resolved X-ray single-molecule internal motions of SARS-CoV-2 spike variants
AU - Sasaki, Daisuke
AU - Arai, Tatsuya
AU - Yang, Yue
AU - Kuramochi, Masahiro
AU - Furuyama, Wakako
AU - Nanbo, Asuka
AU - Sekiguchi, Hiroshi
AU - Morone, Nobuhiro
AU - Mio, Kazuhiro
AU - Sasaki, Yuji C.
N1 - Publisher Copyright:
© 2024 The Authors
PY - 2024/7
Y1 - 2024/7
N2 - Single-molecule intramolecular dynamics were successfully measured for three variants of SARS-CoV-2 spike protein, alpha: B.1.1.7, delta: B.1.617, and omicron: B.1.1.529, with a time resolution of 100 μs using X-rays. The results were then compared with respect to the magnitude and directions of motions for the three variants. The largest 3-D intramolecular movement was observed for the omicron variant irrespective of ACE2 receptor binding. A more detailed analysis of the intramolecular motions revealed that the distribution state of intramolecular motion for the three variants was completely different with and without ACE2 receptor binding. The molecular dynamics for the trimeric spike protein of the omicron variant increased when ACE2 binding occurred. At that time, the diffusion constant increased from 71.0 [mrad2/ms] to 91.1 [mrad2/ms].
AB - Single-molecule intramolecular dynamics were successfully measured for three variants of SARS-CoV-2 spike protein, alpha: B.1.1.7, delta: B.1.617, and omicron: B.1.1.529, with a time resolution of 100 μs using X-rays. The results were then compared with respect to the magnitude and directions of motions for the three variants. The largest 3-D intramolecular movement was observed for the omicron variant irrespective of ACE2 receptor binding. A more detailed analysis of the intramolecular motions revealed that the distribution state of intramolecular motion for the three variants was completely different with and without ACE2 receptor binding. The molecular dynamics for the trimeric spike protein of the omicron variant increased when ACE2 binding occurred. At that time, the diffusion constant increased from 71.0 [mrad2/ms] to 91.1 [mrad2/ms].
KW - Diffracted X-ray tracking
KW - Micro-second time-resolved measurement
KW - SARS-CoV-2 spike protein
KW - Single-molecule internal dynamics
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U2 - 10.1016/j.bbrep.2024.101712
DO - 10.1016/j.bbrep.2024.101712
M3 - Article
AN - SCOPUS:85191413775
SN - 2405-5808
VL - 38
JO - Biochemistry and Biophysics Reports
JF - Biochemistry and Biophysics Reports
M1 - 101712
ER -