TY - JOUR
T1 - Microcrystal delivery by pulsed liquid droplet for serial femtosecond crystallography
AU - Mafuné, Fumitaka
AU - Miyajima, Ken
AU - Tono, Kensuke
AU - Takeda, Yoshihiro
AU - Kohno, Jun Ya
AU - Miyauchi, Naoya
AU - Kobayashi, Jun
AU - Joti, Yasumasa
AU - Nango, Eriko
AU - Iwata, So
AU - Yabashi, Makina
N1 - Funding Information:
The XFEL experiments were conducted on BL3 of SACLA with the approval of the Japan Synchrotron Radiation Research Institute (JASRI; Proposal Nos. 2012A8028, 2012B8035 and 2015A8048). This work was supported by the X-ray Free-Electron Laser Priority Strategy Program (MEXT); JSPS KAKENHI Grant No. 15K05407. The authors would like to thank Dr Takanori Nakane for providing the Cheetah–CrystFEL pipeline for SACLA and the members of
Publisher Copyright:
© 2016 International Union of Crystallography.
PY - 2016
Y1 - 2016
N2 - A liquid-droplet injector has been developed that delivers pristine microcrystals to an X-ray irradiation area for conducting serial femtosecond crystallography (SFX) with an X-ray free-electron laser (XFEL). By finely tuning the pulsed liquid droplets in time and space, a high hit rate of the XFEL pulses to microcrystals in the droplets was achieved for measurements using 5 mm tetragonal lysozyme crystals, which produced 4265 indexable diffraction images in about 30 min. The structure was determined at a resolution of 2.3 Å from <0.3 mg of protein. With further improvements such as reduction of the droplet size, liquid droplets have considerable potential as a crystal carrier for SFX with low sample consumption.
AB - A liquid-droplet injector has been developed that delivers pristine microcrystals to an X-ray irradiation area for conducting serial femtosecond crystallography (SFX) with an X-ray free-electron laser (XFEL). By finely tuning the pulsed liquid droplets in time and space, a high hit rate of the XFEL pulses to microcrystals in the droplets was achieved for measurements using 5 mm tetragonal lysozyme crystals, which produced 4265 indexable diffraction images in about 30 min. The structure was determined at a resolution of 2.3 Å from <0.3 mg of protein. With further improvements such as reduction of the droplet size, liquid droplets have considerable potential as a crystal carrier for SFX with low sample consumption.
KW - Liquid-droplet injector
KW - Lysozyme
KW - Serial femtosecond crystallography
KW - X-ray free-electron laser
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U2 - 10.1107/S2059798316001480
DO - 10.1107/S2059798316001480
M3 - Article
C2 - 27050131
AN - SCOPUS:85017340049
SN - 0907-4449
VL - 72
SP - 520
EP - 523
JO - Acta Crystallographica Section D: Structural Biology
JF - Acta Crystallographica Section D: Structural Biology
IS - 4
ER -