TY - JOUR
T1 - Microdomain formation in model biomembranes
AU - Masui, Tomomi
AU - Imai, Masayuki
AU - Urakami, Naohito
N1 - Funding Information:
The SANS experiment were done under approval of Neutron Scattering Program Advisory Committee of KENS and ISSP. This work was supported by the Sasakawa Scientific Research Grant from The Japan Science Society.
PY - 2006/11/27
Y1 - 2006/11/27
N2 - We have investigated the microdomain formation in small unilamellar vesicles (SUVs) composed of ternary mixture of deuterated DPPC, hydrogenated DOPC and hydrogenated cholesterol using a contrast matching technique of small angle neutron scattering. Above the miscibility temperature we cannot observe the scattering intensity from the SUVs. Thus, DPPC, DOPC and cholesterol are mixed at the molecular level in membranes. However, below the miscibility temperature we observe characteristic scattering profiles with a scattering maximum, which clearly shows that the lateral segregation of lipids takes place. The observed profiles are well described by a model scattering function for a single hemispherical shape domain with the radius of several nm. With decreasing the temperature the scattering intensity increases with keeping their maximum positions, indicating that the domain composition strongly depends on the temperature.
AB - We have investigated the microdomain formation in small unilamellar vesicles (SUVs) composed of ternary mixture of deuterated DPPC, hydrogenated DOPC and hydrogenated cholesterol using a contrast matching technique of small angle neutron scattering. Above the miscibility temperature we cannot observe the scattering intensity from the SUVs. Thus, DPPC, DOPC and cholesterol are mixed at the molecular level in membranes. However, below the miscibility temperature we observe characteristic scattering profiles with a scattering maximum, which clearly shows that the lateral segregation of lipids takes place. The observed profiles are well described by a model scattering function for a single hemispherical shape domain with the radius of several nm. With decreasing the temperature the scattering intensity increases with keeping their maximum positions, indicating that the domain composition strongly depends on the temperature.
KW - Contrast matching technique
KW - Microdomain
KW - Model biomembrane
UR - http://www.scopus.com/inward/record.url?scp=33751330966&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33751330966&partnerID=8YFLogxK
U2 - 10.1016/j.physb.2006.06.099
DO - 10.1016/j.physb.2006.06.099
M3 - Article
AN - SCOPUS:33751330966
SN - 0921-4526
VL - 385-386 I
SP - 821
EP - 823
JO - Physica B: Condensed Matter
JF - Physica B: Condensed Matter
ER -