Micromorphological effect of calcium phosphate coating on compatibility of magnesium alloy with osteoblast

Sachiko Hiromoto, Tomohiko Yamazaki

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)


Octacalcium phosphate (OCP) and hydroxyapatite (HAp) coatings were developed to control the degradation speed and to improve the biocompatibility of biodegradable magnesium alloys. Osteoblast MG-63 was cultured directly on OCP- and HAp-coated Mg-3Al-1Zn (wt%, AZ31) alloy (OCP- and HAp-AZ31) to evaluate cell compatibility. Cell proliferation was remarkably improved with OCP and HAp coatings which reduced the corrosion and prevented the H2O2 generation on Mg alloy substrate. OCP-AZ31 showed sparse distribution of living cell colonies and dead cells. HAp-AZ31 showed dense and homogeneous distribution of living cells, with dead cells localized over and around corrosion pits, some of which were formed underneath the coating. These results demonstrated that cells were dead due to changes in the local environment, and it is necessary to evaluate the local biocompatibility of magnesium alloys. Cell density on HAp-AZ31 was higher than that on OCP-AZ31 although there was not a significant difference in the amount of Mg ions released in medium between OCP- and HAp-AZ31. The outer layer of OCP and HAp coatings consisted of plate-like crystal with a thickness of around 0.1 μm and rod-like crystals with a diameter of around 0.1 μm, respectively, which grew from a continuous inner layer. Osteoblasts formed focal contacts on the tips of plate-like OCP and rod-like HAp crystals, with heights of 2–5 μm. The spacing between OCP tips of 0.8–1.1 μm was wider than that between HAp tips of 0.2–0.3 μm. These results demonstrated that cell proliferation depended on the micromorphology of the coatings which governed spacing of focal contacts. Consequently, HAp coating is suitable for improving cell compatibility and bone-forming ability of the Mg alloy.

Original languageEnglish
Pages (from-to)96-109
Number of pages14
JournalScience and Technology of Advanced Materials
Issue number1
Publication statusPublished - 2017 Jan 1


  • biodegradable
  • calcium phosphate coatings
  • cell adhesion
  • cell proliferation
  • Magnesium
  • surface chemical property
  • topography


Dive into the research topics of 'Micromorphological effect of calcium phosphate coating on compatibility of magnesium alloy with osteoblast'. Together they form a unique fingerprint.

Cite this