@article{12432a8c0b1e4b4a86b06c9ef1be625f,
title = "Microsecond dynamics of an unfolded protein by a line confocal tracking of single molecule fluorescence",
abstract = "We present a new method for high speed tracking of fluorescence time series from single proteins. The method uses a fast sample flow and a modified confocal microscopy, line confocal microscopy, and achieves the time resolution of less than 20 μs. The obtained time series from the B domain of protein A labeled with donor and acceptor fluorophores suggest conformational heterogeneity and dynamic fluctuations in the unfolded state.",
author = "Hiroyuki Oikawa and Yuta Suzuki and Masataka Saito and Kiyoto Kamagata and Munehito Arai and Satoshi Takahashi",
note = "Funding Information: S.T., K.K. and M.A. were supported in part by Grants-in-Aids for Scientific Research from MEXT. S.T. and K.K. were supported in part by the Japan Society for the Promotion of Science (JSPS) through the {"}Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program){"} initiated by the Council for Science and Technology Policy (CSTP). M.A. was supported by Kurata Grant from the Kurata Memorial Hitachi Science and Technology Foundation, by Grant for Basic Science Research Projects from the Sumitomo Foundation, and by Japan Science and Technology Agency, PRESTO.",
year = "2013",
doi = "10.1038/srep02151",
language = "English",
volume = "3",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
}