TY - JOUR
T1 - Migration of Phospholipid Vesicles Can Be Selectively Driven by Concentration Gradients of Metal Chloride Solutions
AU - Kodama, Atsuji
AU - Sakuma, Yuka
AU - Imai, Masayuki
AU - Kawakatsu, Toshihiro
AU - Puff, Nicolas
AU - Angelova, Miglena I.
N1 - Funding Information:
This work was supported by JSPS KAKENHI Grand Numbers, JP16H02216, K325800233, and JSPS KAKENHI ‘‘Fluctuation & Structure’’ Grant Number JP25103009, and the Core-to-Core Program ‘‘Non-equilibrium dynamics of soft matter and information’’ from JSPS.
Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/10/10
Y1 - 2017/10/10
N2 - We have investigated the migrations of phospholipid vesicles under the concentration gradients of metal ions. We microinjected metal chloride solutions, monovalent (NaCl and KCl), divalent (CaCl2 and MgCl2), and trivalent (LaCl3) salts, toward phospholipid giant vesicles (GVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC). For NaCl, CaCl2, and MgCl2 solutions, the GVs migrated straight toward the tip of the micropipette in response to the concentration gradients, whereas for KCl and LaCl3, GVs moved to the opposite direction. Our motion tracking of lipid domains in a vesicle membrane showed no unidirectional flow in the membrane during the vesicle migration, indicating that the Marangoni mechanism is not responsible for the observed vesicle migration. We calculated the diffusiophoretic velocities for symmetric and asymmetrical electrolytes by solving the Stokes' equation numerically. The theoretical diffusiophoretic velocities described the observed migration velocities well. Thus, we can control the migration of vesicle in response to the concentration gradient by adapting the electrolytes and the lipids.
AB - We have investigated the migrations of phospholipid vesicles under the concentration gradients of metal ions. We microinjected metal chloride solutions, monovalent (NaCl and KCl), divalent (CaCl2 and MgCl2), and trivalent (LaCl3) salts, toward phospholipid giant vesicles (GVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC). For NaCl, CaCl2, and MgCl2 solutions, the GVs migrated straight toward the tip of the micropipette in response to the concentration gradients, whereas for KCl and LaCl3, GVs moved to the opposite direction. Our motion tracking of lipid domains in a vesicle membrane showed no unidirectional flow in the membrane during the vesicle migration, indicating that the Marangoni mechanism is not responsible for the observed vesicle migration. We calculated the diffusiophoretic velocities for symmetric and asymmetrical electrolytes by solving the Stokes' equation numerically. The theoretical diffusiophoretic velocities described the observed migration velocities well. Thus, we can control the migration of vesicle in response to the concentration gradient by adapting the electrolytes and the lipids.
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U2 - 10.1021/acs.langmuir.7b02617
DO - 10.1021/acs.langmuir.7b02617
M3 - Article
C2 - 28895737
AN - SCOPUS:85032858500
SN - 0743-7463
VL - 33
SP - 10698
EP - 10706
JO - Langmuir
JF - Langmuir
IS - 40
ER -