TY - JOUR
T1 - Mitogen-activated protein kinases MpkA and MpkB independently affect micafungin sensitivity in Aspergillus nidulans
AU - Yoshimi, Akira
AU - Fujioka, Tomonori
AU - Mizutani, Osamu
AU - Marui, Junichiro
AU - Hagiwara, Daisuke
AU - Abe, Keietsu
N1 - Publisher Copyright:
© 2015 JPN Society for Bioscience, Biotechnology, and Agrochemistry.
PY - 2015
Y1 - 2015
N2 - The transcriptional regulation of the MAPK mpkA and cell wall-related genes in Aspergillus nidulans differs from that of their counterparts in Saccharomyces cerevisiae. The A. nidulans MAPK MpkB is putatively orthologous to the yeast MAPKs Kss1p and Fus3p. To investigate MpkB and its contribution to cell wall integrity in A. nidulans, we constructed mpkB-disruptant (mpkBδ) strains. We previously showed that mpkAδ strains exhibited reduced colony growth and increased sensitivity to the β-1,3-glucan synthase inhibitor micafungin. Like mpkAδ strains, mpkBδ strains exhibited slight growth retardation and increased sensitivity to micafungin. Although MpkB-dependent signaling modulated the transcription of some cell wall-related genes, the sugar composition of cell wall fractions was similar among wild-type, mpkAδ, and mpkBδ strains. To elucidate the relationship between MpkA and MpkB pathways, we compared conditional mutants of mpkB with those with mpkA deletion. Sensitivity testing suggested that MpkA and MpkB additively contribute to micafungin activity in A. nidulans.
AB - The transcriptional regulation of the MAPK mpkA and cell wall-related genes in Aspergillus nidulans differs from that of their counterparts in Saccharomyces cerevisiae. The A. nidulans MAPK MpkB is putatively orthologous to the yeast MAPKs Kss1p and Fus3p. To investigate MpkB and its contribution to cell wall integrity in A. nidulans, we constructed mpkB-disruptant (mpkBδ) strains. We previously showed that mpkAδ strains exhibited reduced colony growth and increased sensitivity to the β-1,3-glucan synthase inhibitor micafungin. Like mpkAδ strains, mpkBδ strains exhibited slight growth retardation and increased sensitivity to micafungin. Although MpkB-dependent signaling modulated the transcription of some cell wall-related genes, the sugar composition of cell wall fractions was similar among wild-type, mpkAδ, and mpkBδ strains. To elucidate the relationship between MpkA and MpkB pathways, we compared conditional mutants of mpkB with those with mpkA deletion. Sensitivity testing suggested that MpkA and MpkB additively contribute to micafungin activity in A. nidulans.
KW - Aspergillus nidulans
KW - Cell wall signaling
KW - Micafungin
KW - Mpka
KW - Mpkb
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U2 - 10.1080/09168451.2014.998619
DO - 10.1080/09168451.2014.998619
M3 - Article
C2 - 25727969
AN - SCOPUS:84942099088
SN - 0916-8451
VL - 79
SP - 836
EP - 844
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 5
ER -