Modeling of hollow‐fiber capillary reactor for the production of L‐Alanine with coenzyme regeneration

Tomoyuki Fujii, Osato Miyawaki, Toshimasa Yano

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Continuous production of L‐alanine with conjugated enzyme systems of alanine dehydrogenase (AlaDH) and lactate dehydrogenase (LDH) or alcohol dehydrogenase (ADH) was carried out with NAD regeneration in an ultrafiltration hollow‐fiber capillary reactor (HFCR) which was proposed as a test bioreactor with very small scale. In the AlaDH/LDH system, pyruvate is the intermediate product for L‐alanine so that an optimal point existed in pyruvate concentration for the production rate of L‐alanine. NAD cycling number of 4850 and L‐alanine productivity of 61.7 mmol/L h were obtained at the best condition. In the AlaDH/ADH system, however, the substrate inhibition in the AlaDH reaction by pyruvate should be considered and the best results of NAD cycling number and L‐alanine productivity were 2700 and 13.5 mmol/L h, respectively. In consideration of concentration distribution and mixing in the axial direction on an HFCR, performance of the reactor was theoretically analyzed with a multistage stirred tank reactor model combined with the kinetic model based on all the elementary reactions involved. Although quantitative discrepancy existed in some cases, the present theoretical model could explain experimental results and is expected to be generally applicable to standard hollow fiber reactors.

Original languageEnglish
Pages (from-to)1166-1172
Number of pages7
JournalBiotechnology and Bioengineering
Issue number10
Publication statusPublished - 1991 Dec 5


  • L‐alanine dehydrogenase
  • NAD regeneration
  • elementary reaction kinetics
  • hollow‐fiber capillary reactor
  • multistage stirred tank reactor model


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