TY - JOUR
T1 - Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937
AU - Kobayashi, H.
AU - Albarracin, L.
AU - Sato, N.
AU - Kanmani, P.
AU - Kober, A. K.M.H.
AU - Ikeda-Ohtsubo, W.
AU - Suda, Y.
AU - Nochi, T.
AU - Aso, H.
AU - Makino, S.
AU - Kano, H.
AU - Ohkawara, S.
AU - Saito, T.
AU - Villena, J.
AU - Kitazawa, H.
N1 - Funding Information:
This study was supported by a Grant-in-Aid for Scientific Research (B) (2) (No.24380146, 16H05019), Challenging Exploratory Research (No. 23658216, 26660216, 16 K15028) and Open Partnership Joint Projects of JSPS Bilateral Joint Research Projects from the Japan Society for the Promotion of Science (JSPS) to Dr. H. Kitazawa. Dr. P. Kanmani and Dr. A.K.M.H. Kober were supported by JSPS (Postdoctoral Fellowship for Foreign Researchers, Program No. 25-03397 and No. 15 F15401, respectively).
Publisher Copyright:
© 2016 Wageningen Academic Publishers.
PY - 2016
Y1 - 2016
N2 - In order to evaluate probiotic strains applicable for the beneficial immunomodulation of the porcine gut (immunobiotics), we previously developed a porcine intestinal epitheliocyte cell line (PIE cells). Here, transcriptomic studies using PIE cells were performed considering that this information would be valuable for understanding the mechanisms involved in the protective activity of the immunobiotic strain Lactobacillus jensenii TL2937 against intestinal inflammatory damage in pigs. In addition, those studies would provide criteria for selecting biomarkers for the screening of new immunobiotic strains. We performed microarray analysis to investigate the transcriptomic response of PIE cells to the challenge with heat-stable enterotoxigenic Escherichia coli (ETEC) pathogen-associated molecular patterns (PAMPs) and, the changes induced by L. jensenii TL2937 in that response. The approach allowed us to obtain a global overview of the immune genes involved in the response of PIE cells to heat-stable ETEC PAMPs. We observed that L. jensenii TL2937 differently modulated gene expression in ETEC PAMPs-challenged PIE cells. Microarray and RT-PCR analysis indicated that the most remarkable changes in PIE cells transcriptomic profile after heat-stable ETEC PAMPs challenge were observed in chemokines, adhesion molecules, complement and coagulation cascades factors. In addition, an anti-inflammatory effect triggered by TL2937 strain in PIE cells was clearly demonstrated. The decrease in the expression of chemokines (CCL8, CXCL5, CXCL9, CXCL10, and CXCL11), complement (C1R, C1S, C3, and CFB), and coagulation factors (F3) by L. jensenii TL2937 supports our previous reports on the immunoregulatory effect of this strain. These results provided clues for the better understanding of the mechanism underlying host-immunobiotic interaction in the porcine host. The comprehensive transcriptomic profiles of PIE cells provided by our analyses successfully identified a group of genes, which could be used as prospective biomarkers for the screening and evaluation of new anti-inflammatory immunobiotics for the prevention of inflammatory intestinal disorders in pigs.
AB - In order to evaluate probiotic strains applicable for the beneficial immunomodulation of the porcine gut (immunobiotics), we previously developed a porcine intestinal epitheliocyte cell line (PIE cells). Here, transcriptomic studies using PIE cells were performed considering that this information would be valuable for understanding the mechanisms involved in the protective activity of the immunobiotic strain Lactobacillus jensenii TL2937 against intestinal inflammatory damage in pigs. In addition, those studies would provide criteria for selecting biomarkers for the screening of new immunobiotic strains. We performed microarray analysis to investigate the transcriptomic response of PIE cells to the challenge with heat-stable enterotoxigenic Escherichia coli (ETEC) pathogen-associated molecular patterns (PAMPs) and, the changes induced by L. jensenii TL2937 in that response. The approach allowed us to obtain a global overview of the immune genes involved in the response of PIE cells to heat-stable ETEC PAMPs. We observed that L. jensenii TL2937 differently modulated gene expression in ETEC PAMPs-challenged PIE cells. Microarray and RT-PCR analysis indicated that the most remarkable changes in PIE cells transcriptomic profile after heat-stable ETEC PAMPs challenge were observed in chemokines, adhesion molecules, complement and coagulation cascades factors. In addition, an anti-inflammatory effect triggered by TL2937 strain in PIE cells was clearly demonstrated. The decrease in the expression of chemokines (CCL8, CXCL5, CXCL9, CXCL10, and CXCL11), complement (C1R, C1S, C3, and CFB), and coagulation factors (F3) by L. jensenii TL2937 supports our previous reports on the immunoregulatory effect of this strain. These results provided clues for the better understanding of the mechanism underlying host-immunobiotic interaction in the porcine host. The comprehensive transcriptomic profiles of PIE cells provided by our analyses successfully identified a group of genes, which could be used as prospective biomarkers for the screening and evaluation of new anti-inflammatory immunobiotics for the prevention of inflammatory intestinal disorders in pigs.
KW - ETEC
KW - Immunobiotic biomarkers
KW - Immunotranscriptomic response
KW - Lactobacillus jensenii TL2937
KW - Porcine intestinal epitheliocytes
UR - http://www.scopus.com/inward/record.url?scp=85006817438&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85006817438&partnerID=8YFLogxK
U2 - 10.3920/BM2016.0095
DO - 10.3920/BM2016.0095
M3 - Article
C2 - 27824278
AN - SCOPUS:85006817438
SN - 1876-2883
VL - 7
SP - 769
EP - 782
JO - Beneficial microbes
JF - Beneficial microbes
IS - 5
ER -