Molecular analysis of cryptosporidium parvum HNJ-1 isolated in Japan

Said Amer, Ryuma Matsubara, Fumi Murakoshi, Yutaka Nakai

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2 Citations (Scopus)


Cryptosporidium parvum HNJ-1 is widely used as a reference strain in Japan. In the present study, the parasite was subjected for further molecular analysis including transcribed ribosomal region (ITS rRNA), dihydrofolate reductase (DHFR) and surface glycoprotein (GP60) genes. Partial sequence analysis of these genes indicated extensive polymorphism in ITS region compared with relevant sequences of other Cryptosporidium parvum isolates. In addition, this strain was identified as C. parvum IIaA15G2R1 subtype, based on the sequence results of GP60 gene locus.

Original languageEnglish
Pages (from-to)1647-1649
Number of pages3
JournalJournal of Veterinary Medical Science
Issue number12
Publication statusPublished - 2010 Dec


  • Cryptosporidium parvum
  • DHFR
  • GP60
  • HNJ-1
  • ITS


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