Molecular analysis of cryptosporidium parvum HNJ-1 isolated in Japan

Said Amer; Ryuma Matsubara; Fumi Murakoshi; Yutaka Nakai

doi:10.1292/jvms.10-0118

Molecular analysis of cryptosporidium parvum HNJ-1 isolated in Japan

Said Amer, Ryuma Matsubara, Fumi Murakoshi, Yutaka Nakai

FRIS - Core Interdisciplinary Research Section

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2 Citations (Scopus)

Abstract

Cryptosporidium parvum HNJ-1 is widely used as a reference strain in Japan. In the present study, the parasite was subjected for further molecular analysis including transcribed ribosomal region (ITS rRNA), dihydrofolate reductase (DHFR) and surface glycoprotein (GP60) genes. Partial sequence analysis of these genes indicated extensive polymorphism in ITS region compared with relevant sequences of other Cryptosporidium parvum isolates. In addition, this strain was identified as C. parvum IIaA15G2R1 subtype, based on the sequence results of GP60 gene locus.

Original language	English
Pages (from-to)	1647-1649
Number of pages	3
Journal	Journal of Veterinary Medical Science
Volume	72
Issue number	12
DOIs	https://doi.org/10.1292/jvms.10-0118
Publication status	Published - 2010 Dec

Keywords

Cryptosporidium parvum
DHFR
GP60
HNJ-1
ITS

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10.1292/jvms.10-0118

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abstract = "Cryptosporidium parvum HNJ-1 is widely used as a reference strain in Japan. In the present study, the parasite was subjected for further molecular analysis including transcribed ribosomal region (ITS rRNA), dihydrofolate reductase (DHFR) and surface glycoprotein (GP60) genes. Partial sequence analysis of these genes indicated extensive polymorphism in ITS region compared with relevant sequences of other Cryptosporidium parvum isolates. In addition, this strain was identified as C. parvum IIaA15G2R1 subtype, based on the sequence results of GP60 gene locus.",

keywords = "Cryptosporidium parvum, DHFR, GP60, HNJ-1, ITS",

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AU - Matsubara, Ryuma

AU - Murakoshi, Fumi

AU - Nakai, Yutaka

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AB - Cryptosporidium parvum HNJ-1 is widely used as a reference strain in Japan. In the present study, the parasite was subjected for further molecular analysis including transcribed ribosomal region (ITS rRNA), dihydrofolate reductase (DHFR) and surface glycoprotein (GP60) genes. Partial sequence analysis of these genes indicated extensive polymorphism in ITS region compared with relevant sequences of other Cryptosporidium parvum isolates. In addition, this strain was identified as C. parvum IIaA15G2R1 subtype, based on the sequence results of GP60 gene locus.

KW - Cryptosporidium parvum

KW - DHFR

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KW - ITS

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Molecular analysis of cryptosporidium parvum HNJ-1 isolated in Japan

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Keywords

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