Molecular and functional characterization of a rose lipoxygenase cDNA related to flower senescence

Masako Fukuchi-Mizutani; Kanako Ishiguro; Toru Nakayama; Yukiko Utsunomiya; Yoshikazu Tanaka; Takaaki Kusumi; Takashi Ueda

doi:10.1016/S0168-9452(00)00373-3

Molecular and functional characterization of a rose lipoxygenase cDNA related to flower senescence

Masako Fukuchi-Mizutani, Kanako Ishiguro, Toru Nakayama, Yukiko Utsunomiya, Yoshikazu Tanaka, Takaaki Kusumi, Takashi Ueda

ENG - Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

55 Citations (Scopus)

Abstract

A cDNA encoding lipoxygenase, Rlox1, was isolated from a cDNA library of senescing rose petals using tomato lipoxygenase cDNA fragments as probes. Characterization of the Rlox1 protein expressed in Echerichia coli revealed that the Rlox1 protein was a soluble lipoxygenase with an unusual optimal pH in the acidic region (pH 4.5-5.0). Northern blot analysis showed that the transcript of the Rlox1 gene was dramatically increased in response to senescence of rose petals. Treatment of rose flowers with ethylene also elevated the mRNA of the Rlox1 gene. These results suggest that the Rlox1 lipoxygenase is involved in senescence of rose flowers.

Original language	English
Pages (from-to)	129-137
Number of pages	9
Journal	Plant Science
Volume	160
Issue number	1
DOIs	https://doi.org/10.1016/S0168-9452(00)00373-3
Publication status	Published - 2000 Dec 7

Keywords

Ethylene
Flower senescence
Lipoxygenase
Rosa hybrida

Access to Document

10.1016/S0168-9452(00)00373-3

Cite this

@article{e419a4f98c5b4deb9a44261940120fa6,

title = "Molecular and functional characterization of a rose lipoxygenase cDNA related to flower senescence",

abstract = "A cDNA encoding lipoxygenase, Rlox1, was isolated from a cDNA library of senescing rose petals using tomato lipoxygenase cDNA fragments as probes. Characterization of the Rlox1 protein expressed in Echerichia coli revealed that the Rlox1 protein was a soluble lipoxygenase with an unusual optimal pH in the acidic region (pH 4.5-5.0). Northern blot analysis showed that the transcript of the Rlox1 gene was dramatically increased in response to senescence of rose petals. Treatment of rose flowers with ethylene also elevated the mRNA of the Rlox1 gene. These results suggest that the Rlox1 lipoxygenase is involved in senescence of rose flowers.",

keywords = "Ethylene, Flower senescence, Lipoxygenase, Rosa hybrida",

author = "Masako Fukuchi-Mizutani and Kanako Ishiguro and Toru Nakayama and Yukiko Utsunomiya and Yoshikazu Tanaka and Takaaki Kusumi and Takashi Ueda",

year = "2000",

month = dec,

day = "7",

doi = "10.1016/S0168-9452(00)00373-3",

language = "English",

volume = "160",

pages = "129--137",

journal = "Plant Science",

issn = "0168-9452",

publisher = "Elsevier Ireland Ltd",

number = "1",

}

TY - JOUR

T1 - Molecular and functional characterization of a rose lipoxygenase cDNA related to flower senescence

AU - Fukuchi-Mizutani, Masako

AU - Ishiguro, Kanako

AU - Nakayama, Toru

AU - Utsunomiya, Yukiko

AU - Tanaka, Yoshikazu

AU - Kusumi, Takaaki

AU - Ueda, Takashi

PY - 2000/12/7

Y1 - 2000/12/7

N2 - A cDNA encoding lipoxygenase, Rlox1, was isolated from a cDNA library of senescing rose petals using tomato lipoxygenase cDNA fragments as probes. Characterization of the Rlox1 protein expressed in Echerichia coli revealed that the Rlox1 protein was a soluble lipoxygenase with an unusual optimal pH in the acidic region (pH 4.5-5.0). Northern blot analysis showed that the transcript of the Rlox1 gene was dramatically increased in response to senescence of rose petals. Treatment of rose flowers with ethylene also elevated the mRNA of the Rlox1 gene. These results suggest that the Rlox1 lipoxygenase is involved in senescence of rose flowers.

AB - A cDNA encoding lipoxygenase, Rlox1, was isolated from a cDNA library of senescing rose petals using tomato lipoxygenase cDNA fragments as probes. Characterization of the Rlox1 protein expressed in Echerichia coli revealed that the Rlox1 protein was a soluble lipoxygenase with an unusual optimal pH in the acidic region (pH 4.5-5.0). Northern blot analysis showed that the transcript of the Rlox1 gene was dramatically increased in response to senescence of rose petals. Treatment of rose flowers with ethylene also elevated the mRNA of the Rlox1 gene. These results suggest that the Rlox1 lipoxygenase is involved in senescence of rose flowers.

KW - Ethylene

KW - Flower senescence

KW - Lipoxygenase

KW - Rosa hybrida

UR - http://www.scopus.com/inward/record.url?scp=0034619732&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034619732&partnerID=8YFLogxK

U2 - 10.1016/S0168-9452(00)00373-3

DO - 10.1016/S0168-9452(00)00373-3

M3 - Article

AN - SCOPUS:0034619732

SN - 0168-9452

VL - 160

SP - 129

EP - 137

JO - Plant Science

JF - Plant Science

IS - 1

ER -

Molecular and functional characterization of a rose lipoxygenase cDNA related to flower senescence

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this