Molecular breeding of tomato lines for mass production of miraculin in a plant factory

Kazuhisa Kato, Riichiro Yoshida, Ayako Kikuzaki, Tadayoshi Hirai, Hirofumi Kuroda, Kyoko Hiwasa-Tanase, Kenichi Takane, Hiroshi Ezura, Tsuyoshi Mizoguchi

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41 Citations (Scopus)


A transgenic tomato line (56B, "Moneymaker") that expresses the miraculin gene driven by the CaMV 35S promoter was crossed with a dwarf tomato ("Micro-Tom") for the molecular breeding of cultivars that are suitable for miraculin production in a closed cultivation system. Plant size, miraculin accumulation, and self-pruning growth were used as selection indicators for F2 plants. Two lines were chosen for further analysis, bred to the F6 or F7 generation and cultivated in a closed cultivation system. In 56B and the two crossed lines, the concentrations of miraculin in the pericarp were 140, 367, and 343 μg/g FW, respectively. We also estimated that 26.2, 73.6, and 45.9 kg FW/m2 of tomatoes and 2.2, 16.6, and 9.8 mg/m2 of miraculin in the pericarp, respectively, could be harvested per year. These two crossed lines will be useful for the mass production of miraculin, especially in a closed cultivation system.

Original languageEnglish
Pages (from-to)9505-9510
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Issue number17
Publication statusPublished - 2010 Sept 8


  • Crossbreeding
  • fruit yield
  • miraculin
  • taste-modifying protein
  • tomato


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