TY - JOUR
T1 - Molecular characterization and expression of the cholesteryl ester transfer protein gene in chickens
AU - Sato, Kan
AU - Ohuchi, Aya
AU - Sato, Tomokazu
AU - Schneider, Wolfgang J.
AU - Akiba, Yukio
N1 - Funding Information:
This work was partly supported by Grants-in-Aid (Nos. 14760175 and 15208026) from the Ministry of Education, Science and Culture of Japan, and from the Austrian Science Fund (FWF) to W.J.S.
PY - 2007/10
Y1 - 2007/10
N2 - The cDNA for cholesteryl ester transfer protein (CETP), a protein that catalyzes cholesteryl ester transfer between very low density and high density lipoproteins in plasma, was isolated from chicken liver. When the recombinant protein was overexpressed in HEK293 cells, cholesteryl ester transfer activity was observed in media and cell lysates. By Northern blot analysis, chicken CETP mRNA expression was detected in liver, brain, heart, and spleen. Changes in chicken CETP mRNA expression and plasma CETP activity with nutritional state were examined and found to increase following dietary supplementation with cholesterol in a similar way as in humans. Both the hepatic CETP mRNA levels and plasma CETP activity were significantly lower in mature (i.e egg-laying) hens than in immature female chickens, but were unaffected by age in male animals. Similar changes to those observed in female chickens were observed upon estradiol administration of males. The present study is the first to report the molecular characterization of an avian CETP, and the impairments of CETP gene and activity, which might be regulated by estrogen, play an important role in egg production in laying hens, demonstrating species-specific differences in the lipid metabolism of avian and mammalian species.
AB - The cDNA for cholesteryl ester transfer protein (CETP), a protein that catalyzes cholesteryl ester transfer between very low density and high density lipoproteins in plasma, was isolated from chicken liver. When the recombinant protein was overexpressed in HEK293 cells, cholesteryl ester transfer activity was observed in media and cell lysates. By Northern blot analysis, chicken CETP mRNA expression was detected in liver, brain, heart, and spleen. Changes in chicken CETP mRNA expression and plasma CETP activity with nutritional state were examined and found to increase following dietary supplementation with cholesterol in a similar way as in humans. Both the hepatic CETP mRNA levels and plasma CETP activity were significantly lower in mature (i.e egg-laying) hens than in immature female chickens, but were unaffected by age in male animals. Similar changes to those observed in female chickens were observed upon estradiol administration of males. The present study is the first to report the molecular characterization of an avian CETP, and the impairments of CETP gene and activity, which might be regulated by estrogen, play an important role in egg production in laying hens, demonstrating species-specific differences in the lipid metabolism of avian and mammalian species.
KW - Chicken
KW - Cholesteryl ester transfer protein (CETP)
KW - Estrogen
KW - mRNA expression
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U2 - 10.1016/j.cbpb.2007.05.003
DO - 10.1016/j.cbpb.2007.05.003
M3 - Article
C2 - 17574888
AN - SCOPUS:34547588240
SN - 1096-4959
VL - 148
SP - 117
EP - 123
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
IS - 2
ER -