TY - JOUR
T1 - Molecular characterization of human and rat organic anion transporter OATP-D
AU - Adachi, Hisanobu
AU - Suzuki, Takehiro
AU - Abe, Michiaki
AU - Asano, Naoki
AU - Mizutamari, Hiroya
AU - Tanemoto, Masayuki
AU - Nishio, Toshiyuki
AU - Onogawa, Tohru
AU - Toyohara, Takafumi
AU - Kasai, Satoshi
AU - Satoh, Fumitoshi
AU - Suzuki, Masanori
AU - Tokui, Taro
AU - Unno, Michiaki
AU - Shimosegawa, Tooru
AU - Matsuno, Seiki
AU - Ito, Sadayoshi
AU - Abe, Takaaki
PY - 2003/12
Y1 - 2003/12
N2 - We have isolated and characterized a novel human and rat organic anion transporter subtype, OATP-D. The isolated cDNA from human brain encodes a polypeptide of 710 amino acids (Mr 76,534) with 12 predicted transmembrane domains. The rat clone encodes 710 amino acids (Mr 76,821) with 97.6% amino acid sequence homology with human OATP-D. Human and rat OATP-D have moderate amino acid sequence homology with LST-1/rlst-1, the rat oatp family, the prostaglandin transporter, and moat1/MOAT1/KIAA0880/OATP-B. Phylogenetic tree analysis revealed that OATP-D is branched in a different position from all known organic anion transporters. OATP-D transports prostaglandin E1 (Km 48.5 nM), prostaglandin E 2 (Km 55.5 nM), and prostaglandin F2α, suggesting that, functionally, OATP-D encodes a protein that has similar characteristics to those of the prostaglandin transporter. Rat OATP-D also transports prostaglandins. The expression pattern of OATP-D mRNA was abundant mainly in the heart, testis, brain, and some cancer cells. Immunohistochemical analysis further revealed that rat OATP-D is widely expressed in the vascular, renal, and reproductive system at the protein level. These results suggest that OATP-D plays an important role in translocating prostaglandins in specialized tissues and cells.
AB - We have isolated and characterized a novel human and rat organic anion transporter subtype, OATP-D. The isolated cDNA from human brain encodes a polypeptide of 710 amino acids (Mr 76,534) with 12 predicted transmembrane domains. The rat clone encodes 710 amino acids (Mr 76,821) with 97.6% amino acid sequence homology with human OATP-D. Human and rat OATP-D have moderate amino acid sequence homology with LST-1/rlst-1, the rat oatp family, the prostaglandin transporter, and moat1/MOAT1/KIAA0880/OATP-B. Phylogenetic tree analysis revealed that OATP-D is branched in a different position from all known organic anion transporters. OATP-D transports prostaglandin E1 (Km 48.5 nM), prostaglandin E 2 (Km 55.5 nM), and prostaglandin F2α, suggesting that, functionally, OATP-D encodes a protein that has similar characteristics to those of the prostaglandin transporter. Rat OATP-D also transports prostaglandins. The expression pattern of OATP-D mRNA was abundant mainly in the heart, testis, brain, and some cancer cells. Immunohistochemical analysis further revealed that rat OATP-D is widely expressed in the vascular, renal, and reproductive system at the protein level. These results suggest that OATP-D plays an important role in translocating prostaglandins in specialized tissues and cells.
KW - Eicosanoid
KW - Prostaglandin transporter
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U2 - 10.1152/ajprenal.00402.2002
DO - 10.1152/ajprenal.00402.2002
M3 - Article
C2 - 14631946
AN - SCOPUS:0242497239
SN - 1931-857X
VL - 285
SP - F1188-F1197
JO - American Journal of Physiology - Renal Physiology
JF - American Journal of Physiology - Renal Physiology
IS - 6 54-6
ER -
