Molecular cloning and expression of a rat cDNA encoding MDCK-type chloride channel

Takaaki Abe; Kazuhisa Takeuchi; Kuniaki Ishii; Keishi Abe

doi:10.1016/0167-4781(93)90138-4

Molecular cloning and expression of a rat cDNA encoding MDCK-type chloride channel

Takaaki Abe, Kazuhisa Takeuchi, Kuniaki Ishii, Keishi Abe

MEN - Biomedical Engineering

Tohoku University

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

A cDNA encoding MDCK-type chloride channel was cloned from the rat kidney cDNA library using the homology screening approach. The deduced amino acid sequence of cloned cDNA has a 92.4% homology with the MDCK chloride channel, and electrophysiological analysis using Xenopus oocyte expression system showed the functional character of chloride channel. The rat kidney chloride channel, abbreviated RKCL, is not specific to the kidney, but widely expressed in various tissues. It is suggested that RKCL is important for most cells.

Original language	English
Pages (from-to)	353-356
Number of pages	4
Journal	BBA - Gene Structure and Expression
Volume	1173
Issue number	3
DOIs	https://doi.org/10.1016/0167-4781(93)90138-4
Publication status	Published - 1993 Jun 25

Keywords

(Rat)
(Xenopus)
A7r5
Chloride channel
Gene expression
Oocyte expression

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10.1016/0167-4781(93)90138-4

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title = "Molecular cloning and expression of a rat cDNA encoding MDCK-type chloride channel",

abstract = "A cDNA encoding MDCK-type chloride channel was cloned from the rat kidney cDNA library using the homology screening approach. The deduced amino acid sequence of cloned cDNA has a 92.4% homology with the MDCK chloride channel, and electrophysiological analysis using Xenopus oocyte expression system showed the functional character of chloride channel. The rat kidney chloride channel, abbreviated RKCL, is not specific to the kidney, but widely expressed in various tissues. It is suggested that RKCL is important for most cells.",

keywords = "(Rat), (Xenopus), A7r5, Chloride channel, Gene expression, Oocyte expression",

author = "Takaaki Abe and Kazuhisa Takeuchi and Kuniaki Ishii and Keishi Abe",

note = "Funding Information: This work was supportedi n part by a Grant-in-Aid from the Ministry of Education,S ciencea nd Culture, Japan (No. 03454246a) nd by a grant from Ono Phar-maceuticaCl o. Ltd., Tokyo, Japan. We wish to thank Drs. O. Ohnedaa nd N. Yanai for maintenaceo f TKC2 cells.T . Abe is a recipiento f fellowshipfr om the Japan Societyf or the Promotiono f Science.",

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doi = "10.1016/0167-4781(93)90138-4",

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journal = "BBA - Gene Structure and Expression",

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T1 - Molecular cloning and expression of a rat cDNA encoding MDCK-type chloride channel

AU - Abe, Takaaki

AU - Takeuchi, Kazuhisa

AU - Ishii, Kuniaki

AU - Abe, Keishi

N1 - Funding Information: This work was supportedi n part by a Grant-in-Aid from the Ministry of Education,S ciencea nd Culture, Japan (No. 03454246a) nd by a grant from Ono Phar-maceuticaCl o. Ltd., Tokyo, Japan. We wish to thank Drs. O. Ohnedaa nd N. Yanai for maintenaceo f TKC2 cells.T . Abe is a recipiento f fellowshipfr om the Japan Societyf or the Promotiono f Science.

PY - 1993/6/25

Y1 - 1993/6/25

N2 - A cDNA encoding MDCK-type chloride channel was cloned from the rat kidney cDNA library using the homology screening approach. The deduced amino acid sequence of cloned cDNA has a 92.4% homology with the MDCK chloride channel, and electrophysiological analysis using Xenopus oocyte expression system showed the functional character of chloride channel. The rat kidney chloride channel, abbreviated RKCL, is not specific to the kidney, but widely expressed in various tissues. It is suggested that RKCL is important for most cells.

AB - A cDNA encoding MDCK-type chloride channel was cloned from the rat kidney cDNA library using the homology screening approach. The deduced amino acid sequence of cloned cDNA has a 92.4% homology with the MDCK chloride channel, and electrophysiological analysis using Xenopus oocyte expression system showed the functional character of chloride channel. The rat kidney chloride channel, abbreviated RKCL, is not specific to the kidney, but widely expressed in various tissues. It is suggested that RKCL is important for most cells.

KW - (Rat)

KW - (Xenopus)

KW - A7r5

KW - Chloride channel

KW - Gene expression

KW - Oocyte expression

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SN - 0167-4781

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EP - 356

JO - BBA - Gene Structure and Expression

JF - BBA - Gene Structure and Expression

IS - 3

ER -

Molecular cloning and expression of a rat cDNA encoding MDCK-type chloride channel

Abstract

Keywords

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