Molecular cloning and functional analysis of a cDNA coding for human DOPAchrome tautomerase/tyrosinase-related protein-2

Kouji Yokoyama; Hiroyuki Suzuki; Ken ichi Yasumoto; Yasushi Tomita; Shigeki Shibahara

doi:10.1016/0167-4781(94)90292-5

Molecular cloning and functional analysis of a cDNA coding for human DOPAchrome tautomerase/tyrosinase-related protein-2

Kouji Yokoyama, Hiroyuki Suzuki, Ken ichi Yasumoto, Yasushi Tomita, Shigeki Shibahara

LIF - Molecular and Chemical Life Science

Research output: Contribution to journal › Article › peer-review

79 Citations (Scopus)

Abstract

We have cloned the cDNAs encoding tyrosinase-related protein-2 (TRP-2) from a human melanoma cDNA library. Transient expression of the isolated cDNA in HeLa cells established that TRP-2 is DOPAchrome tautomerase. Human TRP-2/DOPAchrome tautomerase is composed of 519 amino acids with a molecular weight of 59 000 and has about 84% identity with the mouse counterpart.

Original language	English
Pages (from-to)	317-321
Number of pages	5
Journal	BBA - Gene Structure and Expression
Volume	1217
Issue number	3
DOIs	https://doi.org/10.1016/0167-4781(94)90292-5
Publication status	Published - 1994 Apr 6

Keywords

DOPAchrome tautomerase
Melanin
TRP-2
cDNA

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/0167-4781(94)90292-5

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title = "Molecular cloning and functional analysis of a cDNA coding for human DOPAchrome tautomerase/tyrosinase-related protein-2",

abstract = "We have cloned the cDNAs encoding tyrosinase-related protein-2 (TRP-2) from a human melanoma cDNA library. Transient expression of the isolated cDNA in HeLa cells established that TRP-2 is DOPAchrome tautomerase. Human TRP-2/DOPAchrome tautomerase is composed of 519 amino acids with a molecular weight of 59 000 and has about 84% identity with the mouse counterpart.",

keywords = "DOPAchrome tautomerase, Melanin, TRP-2, cDNA",

author = "Kouji Yokoyama and Hiroyuki Suzuki and Yasumoto, {Ken ichi} and Yasushi Tomita and Shigeki Shibahara",

note = "Funding Information: We thank Prof. T. Kasuga for providing HMV-II human melanoma cells. This work is supported in part by Grants-in-Aid for Scientific Research on Priority Areas (to S.S.) and for Scientific Research (C) (to Y.T.) from the Ministry of Education, Science and Culture of Japan.",

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doi = "10.1016/0167-4781(94)90292-5",

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TY - JOUR

T1 - Molecular cloning and functional analysis of a cDNA coding for human DOPAchrome tautomerase/tyrosinase-related protein-2

AU - Yokoyama, Kouji

AU - Suzuki, Hiroyuki

AU - Yasumoto, Ken ichi

AU - Tomita, Yasushi

AU - Shibahara, Shigeki

N1 - Funding Information: We thank Prof. T. Kasuga for providing HMV-II human melanoma cells. This work is supported in part by Grants-in-Aid for Scientific Research on Priority Areas (to S.S.) and for Scientific Research (C) (to Y.T.) from the Ministry of Education, Science and Culture of Japan.

PY - 1994/4/6

Y1 - 1994/4/6

N2 - We have cloned the cDNAs encoding tyrosinase-related protein-2 (TRP-2) from a human melanoma cDNA library. Transient expression of the isolated cDNA in HeLa cells established that TRP-2 is DOPAchrome tautomerase. Human TRP-2/DOPAchrome tautomerase is composed of 519 amino acids with a molecular weight of 59 000 and has about 84% identity with the mouse counterpart.

AB - We have cloned the cDNAs encoding tyrosinase-related protein-2 (TRP-2) from a human melanoma cDNA library. Transient expression of the isolated cDNA in HeLa cells established that TRP-2 is DOPAchrome tautomerase. Human TRP-2/DOPAchrome tautomerase is composed of 519 amino acids with a molecular weight of 59 000 and has about 84% identity with the mouse counterpart.

KW - DOPAchrome tautomerase

KW - Melanin

KW - TRP-2

KW - cDNA

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JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

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Molecular cloning and functional analysis of a cDNA coding for human DOPAchrome tautomerase/tyrosinase-related protein-2

Abstract

Keywords

ASJC Scopus subject areas

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