Molecular cloning of a cDNA encoding a pollen extracellular protein as a potential source of a pollen allergen in Brassica rapa

Kinya Toriyama; Kazunori Hanaoka; Takashi Okada; Masao Watanabe

doi:10.1016/S0014-5793(98)00174-4

Molecular cloning of a cDNA encoding a pollen extracellular protein as a potential source of a pollen allergen in Brassica rapa

Kinya Toriyama, Kazunori Hanaoka, Takashi Okada, Masao Watanabe

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

A polyclonal antiserum was raised against the extracellular pollen proteins of Brassica rapa and used for screening the expression cDNA libraries made from immature anthers. We obtained five groups of cDNA clones, including cDNAs similar to PCP1, thioredoxin, and lipid transfer protein (LTP). Recombinant protein of the cDNA clone showing sequence similarity to LTP was demonstrated to bind IgE of a patient allergic to Brassica pollen. The cDNA clone reported here, therefore, represents a novel pollen allergen of Brassica rapa.

Original language	English
Pages (from-to)	234-238
Number of pages	5
Journal	FEBS Letters
Volume	424
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(98)00174-4
Publication status	Published - 1998 Mar 13

Keywords

Anther
Brassica rapa
Immunoglobulin E
Lipid transfer protein
Pollen allergen
cDNA cloning

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(98)00174-4

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title = "Molecular cloning of a cDNA encoding a pollen extracellular protein as a potential source of a pollen allergen in Brassica rapa",

abstract = "A polyclonal antiserum was raised against the extracellular pollen proteins of Brassica rapa and used for screening the expression cDNA libraries made from immature anthers. We obtained five groups of cDNA clones, including cDNAs similar to PCP1, thioredoxin, and lipid transfer protein (LTP). Recombinant protein of the cDNA clone showing sequence similarity to LTP was demonstrated to bind IgE of a patient allergic to Brassica pollen. The cDNA clone reported here, therefore, represents a novel pollen allergen of Brassica rapa.",

keywords = "Anther, Brassica rapa, Immunoglobulin E, Lipid transfer protein, Pollen allergen, cDNA cloning",

author = "Kinya Toriyama and Kazunori Hanaoka and Takashi Okada and Masao Watanabe",

note = "Funding Information: This research was supported in part by Grants-in-Aid for Special Research on Priority Areas (07281101–07281103; Genetic Dissection of Sexual Differentiation and Pollination Process in Higher Plants) from the Ministry of Education, Science, Culture and Sports, Japan, and by a grant for Pioneering Research Projects in Biotechnology from the Ministry of Agriculture, Forestry and Fisheries, Japan.",

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AU - Toriyama, Kinya

AU - Hanaoka, Kazunori

AU - Okada, Takashi

AU - Watanabe, Masao

N1 - Funding Information: This research was supported in part by Grants-in-Aid for Special Research on Priority Areas (07281101–07281103; Genetic Dissection of Sexual Differentiation and Pollination Process in Higher Plants) from the Ministry of Education, Science, Culture and Sports, Japan, and by a grant for Pioneering Research Projects in Biotechnology from the Ministry of Agriculture, Forestry and Fisheries, Japan.

PY - 1998/3/13

Y1 - 1998/3/13

N2 - A polyclonal antiserum was raised against the extracellular pollen proteins of Brassica rapa and used for screening the expression cDNA libraries made from immature anthers. We obtained five groups of cDNA clones, including cDNAs similar to PCP1, thioredoxin, and lipid transfer protein (LTP). Recombinant protein of the cDNA clone showing sequence similarity to LTP was demonstrated to bind IgE of a patient allergic to Brassica pollen. The cDNA clone reported here, therefore, represents a novel pollen allergen of Brassica rapa.

AB - A polyclonal antiserum was raised against the extracellular pollen proteins of Brassica rapa and used for screening the expression cDNA libraries made from immature anthers. We obtained five groups of cDNA clones, including cDNAs similar to PCP1, thioredoxin, and lipid transfer protein (LTP). Recombinant protein of the cDNA clone showing sequence similarity to LTP was demonstrated to bind IgE of a patient allergic to Brassica pollen. The cDNA clone reported here, therefore, represents a novel pollen allergen of Brassica rapa.

KW - Anther

KW - Brassica rapa

KW - Immunoglobulin E

KW - Lipid transfer protein

KW - Pollen allergen

KW - cDNA cloning

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Molecular cloning of a cDNA encoding a pollen extracellular protein as a potential source of a pollen allergen in Brassica rapa

Abstract

Keywords

ASJC Scopus subject areas

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