Molecular cloning of a genomic dna for enolase from aspergillus oryzae

Masayuki Machida, Trini Violeta Jime’nez Gonzale, Lim Kong Boon, Katsuya Gomi, Yoshifumi Jigami

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9 Citations (Scopus)


We have isolated an enolase gene (enoA) from Aspergillus oryzae by heterologous hybridization using the corresponding Saccharomyces cerevisiae ENO2 gene as a probe. A 2.9-kb BglII-fragment contained the entire structural gene enoA including 5′- and 3′- flanking regions. The homology between A. oryzae enoA and S. cerevisiae ENO2 genes is 66.9% when introns are removed. Genomic Southern analysis indicated that there is only one enolase gene in A. oryzae.

Original languageEnglish
Pages (from-to)161-163
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Issue number1
Publication statusPublished - 1996 Jan


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