Molecular cloning of cDNA for sapecin B, an antibacterial protein of Sarcophaga, and its detection in larval brain

Se Rok Lee; Shoichiro Kurata; Shunji Natori

doi:10.1016/0014-5793(95)00717-N

Molecular cloning of cDNA for sapecin B, an antibacterial protein of Sarcophaga, and its detection in larval brain

Se Rok Lee, Shoichiro Kurata, Shunji Natori

PHA - Life and Pharmaceutical Science

The University of Tokyo

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

A cDNA clone for sapecin B, an antibacterial protein of Sarcophaga, was isolated. This cDNA encoded a precursor protein of sapecin B consisting of a signal sequence (24 residues), prosegment (30 residues) and mature sapecin B (34 residues). Sapecin B was synthesized almost exclusively in the fat body when the larval body wall was injured, but the brain of naive larvae was also demonstrated to contain a significant amount of sapecin B. These findings suggested that sapecin B is a bifunctional protein.

Original language	English
Pages (from-to)	485-487
Number of pages	3
Journal	FEBS Letters
Volume	368
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(95)00717-N
Publication status	Published - 1995 Jul 24

Keywords

Antibacterial protein
Brain
cDNA
Potassium channel inhibitor
Sapecin B

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10.1016/0014-5793(95)00717-N

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title = "Molecular cloning of cDNA for sapecin B, an antibacterial protein of Sarcophaga, and its detection in larval brain",

abstract = "A cDNA clone for sapecin B, an antibacterial protein of Sarcophaga, was isolated. This cDNA encoded a precursor protein of sapecin B consisting of a signal sequence (24 residues), prosegment (30 residues) and mature sapecin B (34 residues). Sapecin B was synthesized almost exclusively in the fat body when the larval body wall was injured, but the brain of naive larvae was also demonstrated to contain a significant amount of sapecin B. These findings suggested that sapecin B is a bifunctional protein.",

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author = "Lee, {Se Rok} and Shoichiro Kurata and Shunji Natori",

note = "Funding Information: Acknowledgements. This work was supported by a Grant-in-Aid for Specifically Promoted Research from the Ministry of Education, Science and Culture of Japan.",

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T1 - Molecular cloning of cDNA for sapecin B, an antibacterial protein of Sarcophaga, and its detection in larval brain

AU - Lee, Se Rok

AU - Kurata, Shoichiro

AU - Natori, Shunji

N1 - Funding Information: Acknowledgements. This work was supported by a Grant-in-Aid for Specifically Promoted Research from the Ministry of Education, Science and Culture of Japan.

PY - 1995/7/24

Y1 - 1995/7/24

N2 - A cDNA clone for sapecin B, an antibacterial protein of Sarcophaga, was isolated. This cDNA encoded a precursor protein of sapecin B consisting of a signal sequence (24 residues), prosegment (30 residues) and mature sapecin B (34 residues). Sapecin B was synthesized almost exclusively in the fat body when the larval body wall was injured, but the brain of naive larvae was also demonstrated to contain a significant amount of sapecin B. These findings suggested that sapecin B is a bifunctional protein.

AB - A cDNA clone for sapecin B, an antibacterial protein of Sarcophaga, was isolated. This cDNA encoded a precursor protein of sapecin B consisting of a signal sequence (24 residues), prosegment (30 residues) and mature sapecin B (34 residues). Sapecin B was synthesized almost exclusively in the fat body when the larval body wall was injured, but the brain of naive larvae was also demonstrated to contain a significant amount of sapecin B. These findings suggested that sapecin B is a bifunctional protein.

KW - Antibacterial protein

KW - Brain

KW - cDNA

KW - Potassium channel inhibitor

KW - Sapecin B

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Molecular cloning of cDNA for sapecin B, an antibacterial protein of Sarcophaga, and its detection in larval brain

Abstract

Keywords

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