Molecular cloning of cDNA for the 29 kDa proteinase participating in decomposition of the larval fat body during metamorphosis of Sarcophaga peregrina (flesh fly)

Noboru Takahashi; Shoichiro Kurata; Shunji Natori

doi:10.1016/0014-5793(93)81702-2

Molecular cloning of cDNA for the 29 kDa proteinase participating in decomposition of the larval fat body during metamorphosis of Sarcophaga peregrina (flesh fly)

Noboru Takahashi, Shoichiro Kurata, Shunji Natori

PHA - Life and Pharmaceutical Science

The University of Tokyo

Research output: Contribution to journal › Article › peer-review

60 Citations (Scopus)

Abstract

A cDNA clone for the 29 kDa proteinase participating in tissue disintegration during metamorphosis of Sarcophaga was isolated. This proteinase, named Sarcophaga cathepsin B, consisted of 256 amino acid residues, and contained three putative N-glycosylation sites. By comparison with other cathepsins B, its unique substrate specificity was partly explained by Ala at position 248.

Original language	English
Pages (from-to)	153-157
Number of pages	5
Journal	FEBS Letters
Volume	334
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(93)81702-2
Publication status	Published - 1993 Nov 15

Keywords

Cathepsin B
cDNA cloning
Fat body decomposition
Metamorphosis

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10.1016/0014-5793(93)81702-2

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title = "Molecular cloning of cDNA for the 29 kDa proteinase participating in decomposition of the larval fat body during metamorphosis of Sarcophaga peregrina (flesh fly)",

abstract = "A cDNA clone for the 29 kDa proteinase participating in tissue disintegration during metamorphosis of Sarcophaga was isolated. This proteinase, named Sarcophaga cathepsin B, consisted of 256 amino acid residues, and contained three putative N-glycosylation sites. By comparison with other cathepsins B, its unique substrate specificity was partly explained by Ala at position 248.",

keywords = "Cathepsin B, cDNA cloning, Fat body decomposition, Metamorphosis",

author = "Noboru Takahashi and Shoichiro Kurata and Shunji Natori",

note = "Funding Information: Acknowledgement. This work was supported by a Grant-in-Aid for Specially Promoted Research from the Ministry of Education, Science and Culture of Japan.",

year = "1993",

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T1 - Molecular cloning of cDNA for the 29 kDa proteinase participating in decomposition of the larval fat body during metamorphosis of Sarcophaga peregrina (flesh fly)

AU - Takahashi, Noboru

AU - Kurata, Shoichiro

AU - Natori, Shunji

N1 - Funding Information: Acknowledgement. This work was supported by a Grant-in-Aid for Specially Promoted Research from the Ministry of Education, Science and Culture of Japan.

PY - 1993/11/15

Y1 - 1993/11/15

N2 - A cDNA clone for the 29 kDa proteinase participating in tissue disintegration during metamorphosis of Sarcophaga was isolated. This proteinase, named Sarcophaga cathepsin B, consisted of 256 amino acid residues, and contained three putative N-glycosylation sites. By comparison with other cathepsins B, its unique substrate specificity was partly explained by Ala at position 248.

AB - A cDNA clone for the 29 kDa proteinase participating in tissue disintegration during metamorphosis of Sarcophaga was isolated. This proteinase, named Sarcophaga cathepsin B, consisted of 256 amino acid residues, and contained three putative N-glycosylation sites. By comparison with other cathepsins B, its unique substrate specificity was partly explained by Ala at position 248.

KW - Cathepsin B

KW - cDNA cloning

KW - Fat body decomposition

KW - Metamorphosis

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JF - FEBS Letters

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ER -

Molecular cloning of cDNA for the 29 kDa proteinase participating in decomposition of the larval fat body during metamorphosis of Sarcophaga peregrina (flesh fly)

Abstract

Keywords

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