Molecular cloning of PP2Cη, a novel member of the protein phosphatase 2C family

Ken Ichiro Komaki; Koji Katsura; Motoko Ohnishi; Ming Guang Li; Masato Sasaki; Makoto Watanabe; Takayasu Kobayashi; Shinri Tamura

doi:10.1016/j.bbaexp.2003.09.004

Molecular cloning of PP2Cη, a novel member of the protein phosphatase 2C family

Ken Ichiro Komaki, Koji Katsura, Motoko Ohnishi, Ming Guang Li, Masato Sasaki, Makoto Watanabe, Takayasu Kobayashi, Shinri Tamura

Center for Laboratory Animal Research (CLAR)

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

We have cloned a novel member of the mouse protein phosphatase 2C family, PP2Cη. Sequence analysis suggests that PP2Cη, PP2Cζ and NERPP-2C constitute a unique subgroup of the PP2C family. PP2Cη had extremely low activity against α-casein compared with PP2Cα and was localized mainly in cell nuclei, suggesting that PP2Cη dephosphorylates a unique nuclear protein(s) in the cells.

Original language	English
Pages (from-to)	130-137
Number of pages	8
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1630
Issue number	2-3
DOIs	https://doi.org/10.1016/j.bbaexp.2003.09.004
Publication status	Published - 2003 Nov 30

Keywords

Cloning
Enzyme activity
Expression
Motif
Protein phosphatase 2C
Subcellular localization

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10.1016/j.bbaexp.2003.09.004

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title = "Molecular cloning of PP2Cη, a novel member of the protein phosphatase 2C family",

abstract = "We have cloned a novel member of the mouse protein phosphatase 2C family, PP2Cη. Sequence analysis suggests that PP2Cη, PP2Cζ and NERPP-2C constitute a unique subgroup of the PP2C family. PP2Cη had extremely low activity against α-casein compared with PP2Cα and was localized mainly in cell nuclei, suggesting that PP2Cη dephosphorylates a unique nuclear protein(s) in the cells.",

keywords = "Cloning, Enzyme activity, Expression, Motif, Protein phosphatase 2C, Subcellular localization",

author = "Komaki, {Ken Ichiro} and Koji Katsura and Motoko Ohnishi and Li, {Ming Guang} and Masato Sasaki and Makoto Watanabe and Takayasu Kobayashi and Shinri Tamura",

note = "Funding Information: This work was supported in part by a Grant-in-Aid for Scientific Research from Ministry of Education, Culture, Sports, Science and Technology of Japan. We are grateful to Mr. Kimio Konno for technical assistance. We are also grateful to Ms. Yuki Sato for secretarial assistance.",

year = "2003",

month = nov,

day = "30",

doi = "10.1016/j.bbaexp.2003.09.004",

language = "English",

volume = "1630",

pages = "130--137",

journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",

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publisher = "Elsevier BV",

number = "2-3",

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TY - JOUR

T1 - Molecular cloning of PP2Cη, a novel member of the protein phosphatase 2C family

AU - Komaki, Ken Ichiro

AU - Katsura, Koji

AU - Ohnishi, Motoko

AU - Li, Ming Guang

AU - Sasaki, Masato

AU - Watanabe, Makoto

AU - Kobayashi, Takayasu

AU - Tamura, Shinri

N1 - Funding Information: This work was supported in part by a Grant-in-Aid for Scientific Research from Ministry of Education, Culture, Sports, Science and Technology of Japan. We are grateful to Mr. Kimio Konno for technical assistance. We are also grateful to Ms. Yuki Sato for secretarial assistance.

PY - 2003/11/30

Y1 - 2003/11/30

N2 - We have cloned a novel member of the mouse protein phosphatase 2C family, PP2Cη. Sequence analysis suggests that PP2Cη, PP2Cζ and NERPP-2C constitute a unique subgroup of the PP2C family. PP2Cη had extremely low activity against α-casein compared with PP2Cα and was localized mainly in cell nuclei, suggesting that PP2Cη dephosphorylates a unique nuclear protein(s) in the cells.

AB - We have cloned a novel member of the mouse protein phosphatase 2C family, PP2Cη. Sequence analysis suggests that PP2Cη, PP2Cζ and NERPP-2C constitute a unique subgroup of the PP2C family. PP2Cη had extremely low activity against α-casein compared with PP2Cα and was localized mainly in cell nuclei, suggesting that PP2Cη dephosphorylates a unique nuclear protein(s) in the cells.

KW - Cloning

KW - Enzyme activity

KW - Expression

KW - Motif

KW - Protein phosphatase 2C

KW - Subcellular localization

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U2 - 10.1016/j.bbaexp.2003.09.004

DO - 10.1016/j.bbaexp.2003.09.004

M3 - Article

AN - SCOPUS:0345118287

SN - 0167-4781

VL - 1630

SP - 130

EP - 137

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

IS - 2-3

ER -

Molecular cloning of PP2Cη, a novel member of the protein phosphatase 2C family

Abstract

Keywords

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