Molecular cloning of rat cDNA for cytosolic phospholipase A2 and the increased gene expression in the dentate gyrus following transient forebrain ischemia

Yuji Owada; Teiji Tominaga; Takashi Yoshimoto; Hisatake Kondo

doi:10.1016/0169-328X(94)90174-0

Molecular cloning of rat cDNA for cytosolic phospholipase A₂ and the increased gene expression in the dentate gyrus following transient forebrain ischemia

Yuji Owada, Teiji Tominaga, Takashi Yoshimoto, Hisatake Kondo

Tohoku University

Research output: Contribution to journal › Article › peer-review

69 Citations (Scopus)

Abstract

Rat cytosolic phospholipase A₂ (cPLA₂) cDNA was cloned from rat brain. The cDNA showed a high homology of 90% in the nucleotide sequence of the coding region with the human counterpart. By in situ hybridization, the gene expression for cPLA₂ was detected in the hippocampus, olfactory bulb, and cerebellar granular cells at very low level of normal rats. The expression was markedly increased in the dentate granule cells of postischemic rat brain. This alteration of the gene expression was discussed in relation to the free fatty acid-mediated neurotoxicity.

Original language	English
Pages (from-to)	364-368
Number of pages	5
Journal	Molecular Brain Research
Volume	25
Issue number	3-4
DOIs	https://doi.org/10.1016/0169-328X(94)90174-0
Publication status	Published - 1994 Sept

Keywords

cDNA cloning
Ischemia
Phospholipase A
Rat brain

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10.1016/0169-328X(94)90174-0

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title = "Molecular cloning of rat cDNA for cytosolic phospholipase A2 and the increased gene expression in the dentate gyrus following transient forebrain ischemia",

abstract = "Rat cytosolic phospholipase A2 (cPLA2) cDNA was cloned from rat brain. The cDNA showed a high homology of 90% in the nucleotide sequence of the coding region with the human counterpart. By in situ hybridization, the gene expression for cPLA2 was detected in the hippocampus, olfactory bulb, and cerebellar granular cells at very low level of normal rats. The expression was markedly increased in the dentate granule cells of postischemic rat brain. This alteration of the gene expression was discussed in relation to the free fatty acid-mediated neurotoxicity.",

keywords = "cDNA cloning, Ischemia, Phospholipase A, Rat brain",

author = "Yuji Owada and Teiji Tominaga and Takashi Yoshimoto and Hisatake Kondo",

note = "Funding Information: This work was supported by Grants in Aid 0667001 from the Ministry of Education, Science and Culture in Japan to H.K., and by a grant from the Tanaka Orthopedic Clinic Foundation, Hamamatsu, Japan to H.K.",

year = "1994",

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T1 - Molecular cloning of rat cDNA for cytosolic phospholipase A2 and the increased gene expression in the dentate gyrus following transient forebrain ischemia

AU - Owada, Yuji

AU - Tominaga, Teiji

AU - Yoshimoto, Takashi

AU - Kondo, Hisatake

N1 - Funding Information: This work was supported by Grants in Aid 0667001 from the Ministry of Education, Science and Culture in Japan to H.K., and by a grant from the Tanaka Orthopedic Clinic Foundation, Hamamatsu, Japan to H.K.

PY - 1994/9

Y1 - 1994/9

N2 - Rat cytosolic phospholipase A2 (cPLA2) cDNA was cloned from rat brain. The cDNA showed a high homology of 90% in the nucleotide sequence of the coding region with the human counterpart. By in situ hybridization, the gene expression for cPLA2 was detected in the hippocampus, olfactory bulb, and cerebellar granular cells at very low level of normal rats. The expression was markedly increased in the dentate granule cells of postischemic rat brain. This alteration of the gene expression was discussed in relation to the free fatty acid-mediated neurotoxicity.

AB - Rat cytosolic phospholipase A2 (cPLA2) cDNA was cloned from rat brain. The cDNA showed a high homology of 90% in the nucleotide sequence of the coding region with the human counterpart. By in situ hybridization, the gene expression for cPLA2 was detected in the hippocampus, olfactory bulb, and cerebellar granular cells at very low level of normal rats. The expression was markedly increased in the dentate granule cells of postischemic rat brain. This alteration of the gene expression was discussed in relation to the free fatty acid-mediated neurotoxicity.

KW - cDNA cloning

KW - Ischemia

KW - Phospholipase A

KW - Rat brain

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Molecular cloning of rat cDNA for cytosolic phospholipase A₂ and the increased gene expression in the dentate gyrus following transient forebrain ischemia

Abstract

Keywords

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