Molecular cloning of the nemA gene encoding N-ethylmaleimide reductase from Escherichia coli

Koichi Miura; Yoshihisa Tomioka; Hideaki Suzuki; Motoko Yonezawa; Takanori Hishinuma; Michinao Mizugaki

doi:10.1248/bpb.20.110

Molecular cloning of the nemA gene encoding N-ethylmaleimide reductase from Escherichia coli

Koichi Miura, Yoshihisa Tomioka, Hideaki Suzuki, Motoko Yonezawa, Takanori Hishinuma, Michinao Mizugaki

PHA - Pharmacy

Tohoku University

Research output: Contribution to journal › Article › peer-review

77 Citations (Scopus)

Abstract

Using the gene mapping membrane technique, we identified a gene (nemA) that encodes N-ethylmaleimide reductase in Escherichia coli. The open reading frame encodes a polypeptide of 365 amino acids with a molecular mass of 39,514 Da. The deduced amino acid sequence showed a high degree of homology (87% identical) with the pentaerythritol tetranitrate reductase of Enterobacter cloacae and the morphinone reductase of Pseudomonasputida (52% identical).

Original language	English
Pages (from-to)	110-112
Number of pages	3
Journal	Biological and Pharmaceutical Bulletin
Volume	20
Issue number	1
DOIs	https://doi.org/10.1248/bpb.20.110
Publication status	Published - 1997 Jan

Keywords

Escherichia coli
N-ethylmaleimide reductase
Old Yellow Enzyme
unsaturated fatty acid

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AU - Mizugaki, Michinao

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KW - Old Yellow Enzyme

KW - unsaturated fatty acid

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Molecular cloning of the nemA gene encoding N-ethylmaleimide reductase from Escherichia coli

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Keywords

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