Molecular dissection of α- and dense-core granule secretion of platelets

Akira Yoshioka, Hisanori Horiuchi, Ryutaro Shirakawa, Hiroaki Nishioka, Arata Tabuchi, Tomohito Higashi, Akitsugu Yamamoto, Toru Kita

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Upon activation, platelets release many active substances stored in α- and dense-core granules. However, the molecular mechanisms governing the regulated exocytosis are not yet fully understood. We have established an assay system using streptolysin-O-permeabilized platelets to analyze the Ca2+-induced secretions of von Willebrand factor stored in α-granules and [3H]5-hydroxytryptamine (5-HT) in dense-core granules. Using the assay, we found that small GTPase Rab4 regulates α-, but not dense-core, granule secretion in platelets. Furthermore, we purified a cytosolic essential protein and currently are analyzing its function.

Original languageEnglish
Pages (from-to)403-406
Number of pages4
JournalAnnals of the New York Academy of Sciences
Publication statusPublished - 2001


  • Dense-core granules
  • Molecular dissection
  • Platelet secretion
  • α-granules


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