@article{5ca816ecb446481b8d478bc088eb91c0,
title = "Molecular phylogeny and taxonomy of the Hydrangea serrata complex (Hydrangeaceae) in western Japan, including a new subspecies of H. acuminata from Yakushima",
abstract = "According to the contemporary classification of Hydrangea native to Japan, H. serrata is a polymorphic species including six varieties. We discovered a plant identified as H. serrata, but morphologically distinct from previously known varieties, in Yakushima island where approximately 50 endemic species are known. To determine the relationship of this plant with previously known varieties, we examined morphology and constructed a highly resolved phylogeny of H. serrata and its relatives using three chloroplast genomic regions, rbcL, trnL intron, psbA-trnH, and two nuclear genomic regions, ITS1 and ITS2, and Multiplex ISSR genotyping by sequencing (MIG-seq). Based on these morphological and phylogenetic observations, we describe Hydrangea acuminata subsp. yakushimensis subsp. nov. as a newly discovered lineage in Yakushima, Japan and propose Hydrangea minamitanii stat. nov. and Hydrangea acuminata subsp. australis stat. nov. which were previously treated as varieties of H. serrata.",
keywords = "Cpdna, Dna barcoding, Fst, Island, Its, Mig-seq, Threatened plants",
author = "Hirota, {Shun K.} and Tetsukazu Yahara and Kengo Fuse and Hiroyuki Sato and Shuichiro Tagane and Shinji Fujii and Tadashi Minamitani and Yoshihisa Suyama",
note = "Funding Information: We thank Toshihiro Saito for his help with our fieldwork in Yakushima. Specimens of H. acuminata subsp. yakushimensis were collected in the protected area of the Yakush-ima National Park with permission from the Ministry of Environment, Yakushima office of Forestry Agency, and Kagoshima office of Agency for Cultural Affairs. Specimens of H. minamitanii, H. luteovenosa, and H. scandens were collected in the protected area of Osuzu Prefectural Natural Park with permission from the Miyazaki Prefecture and Forestry Agency. In addition, specimens of other species were collected in the following national parks with permission from the Ministry of Environment and the prefectures for both Kokuritu and Kokutei Park and in the national forests with permission from the local offices of Forestry Agency: Mt. Shiraiwa of Kyushu-chuo-sanchi National (Kokutei) Park, Mt. Oyaji of Sobo-katamuki National (Ko-kutei) Park, Mt. Karakuni of Kirishima National (Kokuritsu) Park, Osugi-dani of Yoshino-kumano National (Kokuritsu) Park, and Mt. Amagi of Fuji-hakone National (Kokuritu) Park. We thank the Ministry of Environment{\textquoteright}s Rare Species Conservation Promotion Office for their help in obtaining collection permits. We would like to thank Editage (www.editage.com) for English language editing. This study was supported by the Environment Research and Technology Development Fund (JP-MEERF20204001) of the Ministry of the Environment, Japan, and partly by JSPS KAKENHI grant number 21K06307. Funding Information: We thank Toshihiro Saito for his help with our fieldwork in Yakushima. Specimens of H. acuminata subsp. yakushimensis were collected in the protected area of the Yakushima National Park with permission from the Ministry of Environment, Yakushima office of Forestry Agency, and Kagoshima office of Agency for Cultural Affairs. Specimens of H. minamitanii, H. luteovenosa, and H. scandens were collected in the protected area of Osuzu Prefectural Natural Park with permission from the Miyazaki Prefecture and Forestry Agency. In addition, specimens of other species were collected in the following national parks with permission from the Ministry of Environment and the prefectures for both Kokuritu and Kokutei Park and in the national forests with permission from the local offices of Forestry Agency: Mt. Shiraiwa of Kyushuchuo-sanchi National (Kokutei) Park, Mt. Oyaji of Sobo-katamuki National (Kokutei) Park, Mt. Karakuni of Kirishima National (Kokuritsu) Park, Osugi-dani of Yoshino-kumano National (Kokuritsu) Park, and Mt. Amagi of Fuji-hakone National (Kokuritu) Park. We thank the Ministry of Environment?s Rare Species Conservation Promotion Office for their help in obtaining collection permits. We would like to thank Editage (www.editage.com) for English language editing. This study was supported by the Environment Research and Technology Development Fund (JPMEERF20204001) of the Ministry of the Environment, Japan, and partly by JSPS KAKENHI grant number 21K06307. Publisher Copyright: {\textcopyright} Shun K. Hirota et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",
year = "2022",
doi = "10.3897/PHYTOKEYS.188.64259",
language = "English",
volume = "188",
pages = "49--71",
journal = "PhytoKeys",
issn = "1314-2011",
publisher = "Pensoft Publishers",
}