The respiration- or photosynthesis-induced change of oxygen concentration was monitored with scanning electrochemical microscopy (SECM). Cell suspension was put into a glass capillary tube and then one edge was sealed with an oxygen permeable membrane. The total volume of the cell suspension in the capillary was as small as 10 μL. The edge of the sealed capillary was imaged with SECM based on oxygen reduction current. The images indicated the respiration activity as low as 2.0×103 HeLa-S3 cells. This method was also applied for imaging the respiration and photosynthesis activities of a single alga (Bryopsis plumosa) protoplast.
- Living Cell
- Oxygen Electrode
- Scanning Electrochemical Microscopy
- Small Volume Analysis