TY - JOUR
T1 - Monitoring the cellular activity of a cultured single cell by scanning electrochemical microscopy (SECM). A comparison with fluorescence viability monitoring
AU - Kaya, Takatoshi
AU - Torisawa, Yu Suke
AU - Oyamatsu, Daisuke
AU - Nishizawa, Matsuhiko
AU - Matsue, Tomokazu
N1 - Funding Information:
The present work was supported by Grants-in-Aid for Scientific Research on Priority Area (single cell molecular technology, No. 1122720) and B (No. 13450348) from the Ministry of Education, Science and Culture, Japan.
PY - 2003/10/1
Y1 - 2003/10/1
N2 - The respiratory activities of cultured HeLa cells were monitored at a single cell level using scanning electrochemical microscopy (SECM) that produces images of the localized distribution of oxygen around the cell. The change in the cellular activity was traced after exposures to KCN, ethyl alcohol and the antibiotic drug, Antimycin A. The results were compared with those from the conventional fluorescence monitoring using Calcein-AM that is sensitive to deformation of the cell membrane. The SECM-based measurement follows the decrease in the cellular activity upon exposure to KCN and Antimycin A more rapidly than the fluorescence-based measurements, demonstrating that SECM is suitable for studying the cellular influence of respiration inhibitors.
AB - The respiratory activities of cultured HeLa cells were monitored at a single cell level using scanning electrochemical microscopy (SECM) that produces images of the localized distribution of oxygen around the cell. The change in the cellular activity was traced after exposures to KCN, ethyl alcohol and the antibiotic drug, Antimycin A. The results were compared with those from the conventional fluorescence monitoring using Calcein-AM that is sensitive to deformation of the cell membrane. The SECM-based measurement follows the decrease in the cellular activity upon exposure to KCN and Antimycin A more rapidly than the fluorescence-based measurements, demonstrating that SECM is suitable for studying the cellular influence of respiration inhibitors.
KW - Calcein
KW - Cytotoxicity
KW - Respiration activity
KW - Scanning electrochemical microscopy (SECM)
UR - http://www.scopus.com/inward/record.url?scp=0042706487&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0042706487&partnerID=8YFLogxK
U2 - 10.1016/S0956-5663(03)00083-6
DO - 10.1016/S0956-5663(03)00083-6
M3 - Article
C2 - 12896839
AN - SCOPUS:0042706487
SN - 0956-5663
VL - 18
SP - 1379
EP - 1383
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 11
ER -