TY - JOUR
T1 - Monitoring the expression profiles of genes induced by hyperosmotic, high salinity, and oxidative stress and abscisic acid treatment in Arabidopsis cell culture using a full-length cDNA microarray
AU - Takahashi, Seiji
AU - Seki, Motoaki
AU - Ishida, Junko
AU - Satou, Masakazu
AU - Sakurai, Tetsuya
AU - Narusaka, Mari
AU - Kamiya, Asako
AU - Nakajima, Maiko
AU - Enju, Akiko
AU - Akiyama, Kenji
AU - Yamaguchi-Shinozaki, Kazuko
AU - Shinozaki, Kazuo
N1 - Funding Information:
This work was supported in part by a grant for Genome Research from RIKEN, the Program for Promotion of Basic Research Activities for Innovative Biosciences, the Special Coordination Fund of the Science and Technology Agency, and a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MECSST) to K.S. It was also supported in part by a Grant-in-Aid for Scientific Research on Priority Areas (C) ‘Genome Science’ from MECSST to M.S.
PY - 2004/9
Y1 - 2004/9
N2 - Transcriptional regulation in response to hyperosmotic, high-salinity and oxidative stress, and abscisic acid (ABA) treatment in Arabidopsis suspension-cultured cell line T87 was investigated with a cDNA microarray containing 7000 independent full-length Arabidopsis cDNAs. The transcripts of 102, 11, 84 and 73 genes were increased more than 5-fold within 5 h after treatment with 0.5 M mannitol, 0.1 M NaCl, 50 μM ABA and 10 mM H 2O2, respectively. On the other hand, the transcripts of 44, 57, 25 and 34 genes were down-regulated to less than one-third within 5 h after treatment with 0.5 M mannitol, 0.1 M NaCl, 50 μM ABA and 10 mM H 2O2, respectively. Venn diagram analysis revealed 11 genes were induced significantly by mannitol, NaCl, and ABA, indicating crosstalk among these signaling pathways. Comparison of the genes induced by each stress revealed that 32%, 17% and 33% of mannitol-, NaCl- and ABA-inducible genes were also induced by H2O2, indicating the crosstalk between the signaling pathways for osmotic stress and oxidative stress. Although the expression profiles revealed that the T87 cells had most of the regulatory systems seen in Arabidopsis seedlings, the T87 cells did not have one of ABA-dependent signaling pathways.
AB - Transcriptional regulation in response to hyperosmotic, high-salinity and oxidative stress, and abscisic acid (ABA) treatment in Arabidopsis suspension-cultured cell line T87 was investigated with a cDNA microarray containing 7000 independent full-length Arabidopsis cDNAs. The transcripts of 102, 11, 84 and 73 genes were increased more than 5-fold within 5 h after treatment with 0.5 M mannitol, 0.1 M NaCl, 50 μM ABA and 10 mM H 2O2, respectively. On the other hand, the transcripts of 44, 57, 25 and 34 genes were down-regulated to less than one-third within 5 h after treatment with 0.5 M mannitol, 0.1 M NaCl, 50 μM ABA and 10 mM H 2O2, respectively. Venn diagram analysis revealed 11 genes were induced significantly by mannitol, NaCl, and ABA, indicating crosstalk among these signaling pathways. Comparison of the genes induced by each stress revealed that 32%, 17% and 33% of mannitol-, NaCl- and ABA-inducible genes were also induced by H2O2, indicating the crosstalk between the signaling pathways for osmotic stress and oxidative stress. Although the expression profiles revealed that the T87 cells had most of the regulatory systems seen in Arabidopsis seedlings, the T87 cells did not have one of ABA-dependent signaling pathways.
KW - Abiotic stress
KW - Arabidopsis thaliana
KW - Full-length cDNA
KW - T87 cell culture
KW - cDNA microarray
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U2 - 10.1007/s11103-004-2200-0
DO - 10.1007/s11103-004-2200-0
M3 - Article
C2 - 15604727
AN - SCOPUS:19944434202
SN - 0167-4412
VL - 56
SP - 29
EP - 55
JO - Plant Molecular Biology
JF - Plant Molecular Biology
IS - 1
ER -