TY - JOUR
T1 - Monoclonal antibody (5F3) defining renal cell carcinoma-associated antigen disialosyl globopentaosylceramide (V 3NeuAclV 6NeuAcGb5), and distribution pattern of the antigen in tumor and normal tissues
AU - Ito, Akihiro
AU - Saito, Seiichi
AU - Masuko, Takashi
AU - Oh-Eda, Masayoshi
AU - Matsuura, Tetsu
AU - Satoh, Makoto
AU - Nejad, Fatemeh Moosavi
AU - Enomoto, Takemi
AU - Orikasa, Seiichi
AU - Hakomori, Sen Itiroh
N1 - Funding Information:
This study was supported by a Grants-in-aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture of Japan 09470338 (to S.S.) and 1138207 (to T.E.), and by NIH grant CA80054 to S.H. We thank Dr. Toshiko Takahashi for technical assistance, and Dr. Stephen Anderson for scientific editing and preparation of the manuscript.
PY - 2001
Y1 - 2001
N2 - Renal cell carcinoma (RCC) has been characterized by high expression of three types of disialogangliosides: two based on lacto-series type 1 structure (disialosyl Lc 4, GalNAc disialosyl Lc 4), the other based on globo-series structure (disialosyl globopentaosylceramide; disialosyl Gb5). The present study established a mAb, 5F3, directed to disialosyl Gb5. 5F3 was established after immunization with RCC cell line ACHN. The major disialoganglioside antigen isolated from ACHN cells, showing specific reactivity with 5F3, was characterized unequivocally as disialosyl Gb5 (V 3NeuAclV 6-NeuAcGb5) by identification of the core structure as globopentaosylceramide (Gb5) after enzymatic and acid hydrolysis, and by 2-dimensional 1H-NMR spectroscopy. 5F3 does not react with monosialosyl Gb5 (V 3NeuAcGb5), Gb5, or any lactoseries structures. 5F3 strongly stained 19 of 41 cases of primary RCC tissue. It reacted with proximal tubules (but not distal tubules) of kidney, microglial cells of cerebrum and cerebellum, goblet cells of stomach and intestine, smooth muscle of various organs. It did not react with parenchymatous cells of various organs, except for kidney epithelia and prostate stroma. Immunostaining of RCC tissue by mAb 5F3, in combination with staining by other antibodies directed to globo-series and lacto-series structures, has prognostic significance in defining metastatic potential of RCC.
AB - Renal cell carcinoma (RCC) has been characterized by high expression of three types of disialogangliosides: two based on lacto-series type 1 structure (disialosyl Lc 4, GalNAc disialosyl Lc 4), the other based on globo-series structure (disialosyl globopentaosylceramide; disialosyl Gb5). The present study established a mAb, 5F3, directed to disialosyl Gb5. 5F3 was established after immunization with RCC cell line ACHN. The major disialoganglioside antigen isolated from ACHN cells, showing specific reactivity with 5F3, was characterized unequivocally as disialosyl Gb5 (V 3NeuAclV 6-NeuAcGb5) by identification of the core structure as globopentaosylceramide (Gb5) after enzymatic and acid hydrolysis, and by 2-dimensional 1H-NMR spectroscopy. 5F3 does not react with monosialosyl Gb5 (V 3NeuAcGb5), Gb5, or any lactoseries structures. 5F3 strongly stained 19 of 41 cases of primary RCC tissue. It reacted with proximal tubules (but not distal tubules) of kidney, microglial cells of cerebrum and cerebellum, goblet cells of stomach and intestine, smooth muscle of various organs. It did not react with parenchymatous cells of various organs, except for kidney epithelia and prostate stroma. Immunostaining of RCC tissue by mAb 5F3, in combination with staining by other antibodies directed to globo-series and lacto-series structures, has prognostic significance in defining metastatic potential of RCC.
KW - Disialoganglioside
KW - Globo-series
KW - Metastasis
KW - Monoclohal antibody 5F3
KW - Renal cell carcinoma
UR - http://www.scopus.com/inward/record.url?scp=18444379346&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=18444379346&partnerID=8YFLogxK
U2 - 10.1023/A:1016281002344
DO - 10.1023/A:1016281002344
M3 - Article
C2 - 12084983
AN - SCOPUS:18444379346
SN - 0282-0080
VL - 18
SP - 475
EP - 485
JO - Glycoconjugate Journal
JF - Glycoconjugate Journal
IS - 6
ER -