Multi-capillary based optical sensors for highly sensitive protein detection

Yasuhira Okuyama, Takashi Katagiri, Yuji Matsuura

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A fluorescence measuring method based on glass multi-capillary for detecting trace amounts of proteins is proposed. It promises enhancement of sensitivity due to effects of the adsorption area expansion and the longitudinal excitation. The sensitivity behavior of this method was investigated by using biotin-streptavidin binding. According to experimental examinations, it was found that the sensitivity was improved by a factor of 70 from common glass wells. We also confirmed our measuring system could detect 1 pg/mL of streptavidin. These results suggest that multi-capillary has a potential as a high-sensitive biosensor.

Original languageEnglish
Title of host publicationBiomedical Imaging and Sensing Conference
EditorsYasuhiro Awatsuji, Osamu Matoba, Yoshihisa Aizu, Toyohiko Yatagai
PublisherSPIE
ISBN (Electronic)9781510610033
DOIs
Publication statusPublished - 2017 Jan 1
Event3rd Biomedical Imaging and Sensing Conference - Yokohama, Japan
Duration: 2017 Apr 192017 Apr 21

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume10251
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Other

Other3rd Biomedical Imaging and Sensing Conference
Country/TerritoryJapan
CityYokohama
Period17/4/1917/4/21

Keywords

  • Biosensor
  • Fluorescence
  • Immunoassay
  • Multi-Capillary
  • Protein

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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