@article{c35bc563abaa4829ac5d9c3f795483de,
title = "Multibudded tubules formed by COPII on artificial liposomes",
abstract = "COPII-coated vesicles form at the endoplasmic reticulum for cargo transport to the Golgi apparatus. We used in vitro reconstitution to examine the roles of the COPII scaffold in remodeling the shape of a lipid bilayer. Giant Unilamellar Vesicles were examined using fast confocal fluorescence and cryo-electron microscopy in order to avoid separation steps and minimize mechanical manipulation. COPII showed a preference for high curvature structures, but also sufficient flexibility for binding to low curvatures. The COPII proteins induced beads-on-a-string-like constricted tubules, similar to those previously observed in cells. We speculate about a mechanical pathway for vesicle fission from these multibudded COPII-coated tubules, considering the possibility that withdrawal of the Sar1 amphipathic helix upon GTP hydrolysis leads to lipid bilayer destabilization resulting in fission.",
author = "Kirsten Bacia and Eugene Futai and Simone Prinz and Annette Meister and Sebastian Daum and Daniela Glatte and Briggs, {John A.G.} and Randy Schekman",
note = "Funding Information: We thank Bob Lesch and Crystal Chan for help with protein purifications, James Riches for help with cryo-EM, Holly Aaron (MIC, Berkeley) for assistance with confocal and Gerd Hause (Halle) with electron microscopy. We thank Lelio Orci (Faculty of Medicine, University of Geneva) for providing Fig. 3I. We are grateful to current and former members of the labs of Randy Schekman, Jay Groves, George Oster and David Drubin for help and discussions, especially Chao-Wen Wang, Raghuveer Parthasarathy, John Tran, and Bruno Antonny. K.B. acknowledges a postdoctoral fellowship of the Deutsche Akademie der Naturforscher Leopoldina (BMBF-LPD-9901/8-160). K.B., A.M, S.D. and D.G. were supported by the BMBF ZIK program (FKZ 03Z2HN22) and ERDF grant 1241 09 0001. R.S. and K.B. were supported by the Howard Hughes Medical Institute.",
year = "2011",
doi = "10.1038/srep00017",
language = "English",
volume = "1",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
}