Multifunctionalization of Cells with a Self-Assembling Molecule to Enhance Cell Engraftment

Ippei Takashima, Kosuke Kusamori, Hayase Hakariya, Megumi Takashima, Thi Hue Vu, Yuya Mizukami, Naotaka Noda, Yukiya Takayama, Yousuke Katsuda, Shin Ichi Sato, Yoshinobu Takakura, Makiya Nishikawa, Motonari Uesugi

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


Cell-based therapy is a promising approach to restoring lost functions to compromised organs. However, the issue of inefficient cell engraftment remains to be resolved. Herein, we take a chemical approach to facilitate cell engraftment by using self-assembling molecules which modify two cellular traits: cell survival and invasiveness. In this system, the self-assembling molecule induces syndecan-4 clusters on the cellular surface, leading to enhanced cell viability. Further integration with Halo-tag technology provided this self-assembly structure with matrix metalloproteinase-2 to functionalize cells with cell-invasion activity. In vivo experiments showed that the pretreated cells were able to survive injection and then penetrate and engraft into the host tissue, demonstrating that the system enhances cell engraftment. Therefore, cell-surface modification via an alliance between self-assembling molecules and ligation technologies may prove to be a promising method for cell engraftment.

Original languageEnglish
Pages (from-to)775-783
Number of pages9
JournalACS Chemical Biology
Issue number4
Publication statusPublished - 2019 Apr 19
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine


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