TY - JOUR
T1 - Multilocus genetic analysis of Cryptosporidium parvum from Egypt
AU - Amer, Said
AU - Fayed, Masoud
AU - Honma, Hajime
AU - Fukuda, Yasuhiro
AU - Tada, Chica
AU - Nakai, Yutaka
PY - 2010
Y1 - 2010
N2 - Cryptosporidium parvum is a ubiquitous zonootic parasite causing enteritis in man and animals. Cryptosporidium infection was confirmed microscopically in neonatal calves (less than 6 weeks of age) at Kafr El Sheikh Province, Egypt. Multilocus analysis using a wide array of genetic markers was carried out to assess genetic diversity of C. parvum isolates. PCR amplification and partial sequence analysis of 70 kDa heat shock protein, dihydrofolate reductase, alpha-tubulin, elongation factor 1 alpha as well as thrombospondin-related anonymous protein of Cryptosporidium -1, and thrombospondin-related anonymous protein of Cryptosporidium -2 gene markers were achieved. Data indicated that the analyzed isolates belong to C. parvum genotype II with obvious sequence heterogeneity compared with counterparts deposited in Genebank.
AB - Cryptosporidium parvum is a ubiquitous zonootic parasite causing enteritis in man and animals. Cryptosporidium infection was confirmed microscopically in neonatal calves (less than 6 weeks of age) at Kafr El Sheikh Province, Egypt. Multilocus analysis using a wide array of genetic markers was carried out to assess genetic diversity of C. parvum isolates. PCR amplification and partial sequence analysis of 70 kDa heat shock protein, dihydrofolate reductase, alpha-tubulin, elongation factor 1 alpha as well as thrombospondin-related anonymous protein of Cryptosporidium -1, and thrombospondin-related anonymous protein of Cryptosporidium -2 gene markers were achieved. Data indicated that the analyzed isolates belong to C. parvum genotype II with obvious sequence heterogeneity compared with counterparts deposited in Genebank.
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U2 - 10.1007/s00436-010-1967-y
DO - 10.1007/s00436-010-1967-y
M3 - Article
C2 - 20625766
AN - SCOPUS:79952111236
SN - 0932-0113
VL - 107
SP - 1043
EP - 1047
JO - Parasitology Research
JF - Parasitology Research
IS - 5
ER -
