@inbook{635b6836432a436fa13a3c4e0c9d15b8,
title = "Multimodal functional analysis platform: 1. Ultrathin fluorescence endoscope imaging system enables flexible functional brain imaging",
abstract = "To elucidate the expression mechanisms of brain functions, we have developed an ultrathin fluorescence endoscope imaging system (U-FEIS) that can image cells in the brain at any depth while minimizing the invasion. The endoscope part of U-FEIS consists of a GRIN lens and a 10,000-pixel image fiber with a diameter of 450 μm. The specialized microscope of U-FEIS is within 30 cm square and includes lenses and optical filters optimized for the endoscope. Using U-FEIS, we successfully visualized neurons expressing GFP with single-cell resolution and recorded the multineuronal activities in vitro and in vivo. U-FEIS can also perform imaging and optical stimulation simultaneously. Therefore, U-FEIS should be a powerful optical tool in neuroscience research.",
keywords = "Activity imaging, Endoscope, GRIN lens, Image fiber, Imaging system, Multicellular imaging",
author = "Makoto Osanai and Hideki Miwa and Atsushi Tamura and Satomi Kikuta and Yoshio Iguchi and Yuchio Yanagawa and Kazuto Kobayashi and Norihiro Katayama and Tetsu Tanaka and Hajime Mushiake",
note = "Funding Information: Acknowledgments This work was supported by MEXT KAKENHI (Non-linear Neuro-oscillology) JP18H04931 (to MO), JP15H05872 (to YY); MEXT KAKENHI [JP16H06276 (AdAMS); to MO, YY, KK, NK, TT, and HM]; Brain/MINDS [Mapping by Integrated Neurotechnologies for Disease Studies (JP19dm0207001)], AMED (to MO, KK, HM); Program for Revitalization Promotion, JST (to MO); the Matching Planner Program, JST (to MO); “Innovation Inspired by Nature” Research Support Program, SEKISUI CHEMICAL CO. LTD (to MO). We thank Yuji Kohmura, Taro Kojima, and Takehiko Nagai of Lucir Inc. and Daisuke Komada of KYOCERA Corporation for technical assistance. Funding Information: This work was supported by MEXT KAKENHI (Non-linear Neuro-oscillology) JP18H04931 (to MO), JP15H05872 (to YY); MEXT KAKENHI [JP16H06276 (AdAMS); to MO, YY, KK, NK, TT, and HM]; Brain/MINDS [Mapping by Integrated Neurotechnologies for Disease Studies (JP19dm0207001)], AMED (to MO, KK, HM); Program for Revitalization Promotion, JST (to MO); the Matching Planner Program, JST (to MO); “Innovation Inspired by Nature” Research Support Program, SEKISUI CHEMICAL CO. LTD (to MO). We thank Yuji Kohmura, Taro Kojima, and Takehiko Nagai of Lucir Inc. and Daisuke Komada of KYOCERA Corporation for technical assistance. Publisher Copyright: {\textcopyright} Springer Nature Singapore Pte Ltd. 2021.",
year = "2021",
doi = "10.1007/978-981-15-8763-4_31",
language = "English",
series = "Advances in Experimental Medicine and Biology",
publisher = "Springer",
pages = "471--479",
booktitle = "Advances in Experimental Medicine and Biology",
address = "Germany",
}