@article{6901c2636d414b0d8377a8f669d9ddde,
title = "Multiple Oxidative Modifications in the Ophiobolin Biosynthesis: P450 Oxidations Found in Genome Mining",
abstract = "Heterologous expression of four candidate genes found in ophiobolin gene clusters from three fungal strains was employed to elucidate the late-stage biosynthetic pathway of phytotoxin ophiobolin. Expression of oblBAc (cytochrome P450) from the cryptic gene cluster gave unexpected products, and that of oblBBm/oblBEv from the gene cluster of ophiobolin producers, with oblDBm as the transporter, yielded intermediate ophiobolin C through an unusual four-step oxidation process. The observation made in this study may provide a useful guideline for the elucidation of genuine biosynthetic pathways of natural products.",
author = "Koji Narita and Ryota Chiba and Atsushi Minami and Motoichiro Kodama and Isao Fujii and Katsuya Gomi and Hideaki Oikawa",
note = "Funding Information: This work was financially supported by a MEXT research grant for Scientific Research (A) (15H01835) and partly by a grant for Challenging Exploratory Research (15K12737) to H.O. We are grateful to Dr. J. Ishikawa (National Institute of Infectious Diseases) for genomic analysis of E. variecolor GF10 and Profs. S. Kobayashi (Osaka University) and J. Turgeon (Cornell University) for samples of E. variecolor GF10 and B. maydes C5, respectively. Publisher Copyright: {\textcopyright} 2016 American Chemical Society.",
year = "2016",
month = may,
day = "6",
doi = "10.1021/acs.orglett.6b00552",
language = "English",
volume = "18",
pages = "1980--1983",
journal = "Organic Letters",
issn = "1523-7060",
publisher = "American Chemical Society",
number = "9",
}