Multiple traces of monkeypox detected in non-sewered wastewater with sparse sampling from a densely populated metropolitan area in Asia

Dhammika Leshan Wannigama; Mohan Amarasiri; Parichart Hongsing; Cameron Hurst; Charin Modchang; Sudarat Chadsuthi; Suparinthon Anupong; Phatthranit Phattharapornjaroen; Ali Hosseini Rad Ali Hosseini; Stefan Fernandez; Angkana T. Huang; Naris Kueakulpattana; Chanikan Tanasatitchai; Porames Vatanaprasan; Thammakorn Saethang; Sirirat Luk-in; Robin James Storer; Puey Ounjai; Naveen Kumar Devanga Ragupathi; Phitsanuruk Kanthawee; Daisuke Sano; Takashi Furukawa; Kazunari Sei; Asada Leelahavanichkul; Talerngsak Kanjanabuch; Nattiya Hirankarn; Paul G. Higgins; Anthony Kicic; Tanittha Chatsuwan; Alexander D. McLellan; Shuichi Abe

doi:10.1016/j.scitotenv.2022.159816

Multiple traces of monkeypox detected in non-sewered wastewater with sparse sampling from a densely populated metropolitan area in Asia

Dhammika Leshan Wannigama, Mohan Amarasiri, Parichart Hongsing, Cameron Hurst, Charin Modchang, Sudarat Chadsuthi, Suparinthon Anupong, Phatthranit Phattharapornjaroen, Ali Hosseini Rad Ali Hosseini, Stefan Fernandez, Angkana T. Huang, Naris Kueakulpattana, Chanikan Tanasatitchai, Porames Vatanaprasan, Thammakorn Saethang, Sirirat Luk-in, Robin James Storer, Puey Ounjai, Naveen Kumar Devanga Ragupathi, Phitsanuruk KanthaweeDaisuke Sano, Takashi Furukawa, Kazunari Sei, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Nattiya Hirankarn, Paul G. Higgins, Anthony Kicic, Tanittha Chatsuwan, Alexander D. McLellan, Shuichi Abe

Research output: Contribution to journal › Article › peer-review

Abstract

The monkeypox virus is excreted in the feces of infected individuals. Therefore, there is an interest in using viral load detection in wastewater for sentinel early surveillance at a community level and as a complementary approach to syndromic surveillance. We collected wastewater from 63 sewered and non-sewered locations in Bangkok city center between May and August 2022. Monkeypox viral DNA copy numbers were quantified using real-time polymerase chain reaction (PCR) and confirmed positive by Sanger sequencing. Monkeypox viral DNA was first detected in wastewater from the second week of June 2022, with a mean copy number of 16.4 copies/ml (n = 3). From the first week of July, the number of viral DNA copies increased to a mean copy number of 45.92 copies/ml. Positive samples were Sanger sequenced and confirmed the presence of the monkeypox virus. Our study is the first to detect monkeypox viral DNA in wastewater from various locations within Thailand. Results suggest that this could be a complementary source for detecting viral DNA and predicting upcoming outbreaks.

Original language	English
Article number	159816
Journal	Science of the Total Environment
Volume	858
DOIs	https://doi.org/10.1016/j.scitotenv.2022.159816
Publication status	Published - 2023 Feb 1
Externally published	Yes

Keywords

Monkeypox
Monkeypox Asia
Monkeypox viral DNA
Monkeypox virus in wastewater
WBE
Wastewater surveillance

ASJC Scopus subject areas

Environmental Engineering
Environmental Chemistry
Waste Management and Disposal
Pollution

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10.1016/j.scitotenv.2022.159816

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Wannigama, D. L., Amarasiri, M., Hongsing, P., Hurst, C., Modchang, C., Chadsuthi, S., Anupong, S., Phattharapornjaroen, P., Ali Hosseini, A. H. R., Fernandez, S., Huang, A. T., Kueakulpattana, N., Tanasatitchai, C., Vatanaprasan, P., Saethang, T., Luk-in, S., Storer, R. J., Ounjai, P., Ragupathi, N. K. D., ... Abe, S. (2023). Multiple traces of monkeypox detected in non-sewered wastewater with sparse sampling from a densely populated metropolitan area in Asia. Science of the Total Environment, 858, [159816]. https://doi.org/10.1016/j.scitotenv.2022.159816

Wannigama, DL, Amarasiri, M, Hongsing, P, Hurst, C, Modchang, C, Chadsuthi, S, Anupong, S, Phattharapornjaroen, P, Ali Hosseini, AHR, Fernandez, S, Huang, AT, Kueakulpattana, N, Tanasatitchai, C, Vatanaprasan, P, Saethang, T, Luk-in, S, Storer, RJ, Ounjai, P, Ragupathi, NKD, Kanthawee, P, Sano, D, Furukawa, T, Sei, K, Leelahavanichkul, A, Kanjanabuch, T, Hirankarn, N, Higgins, PG, Kicic, A, Chatsuwan, T, McLellan, AD & Abe, S 2023, 'Multiple traces of monkeypox detected in non-sewered wastewater with sparse sampling from a densely populated metropolitan area in Asia', Science of the Total Environment, vol. 858, 159816. https://doi.org/10.1016/j.scitotenv.2022.159816

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title = "Multiple traces of monkeypox detected in non-sewered wastewater with sparse sampling from a densely populated metropolitan area in Asia",

abstract = "The monkeypox virus is excreted in the feces of infected individuals. Therefore, there is an interest in using viral load detection in wastewater for sentinel early surveillance at a community level and as a complementary approach to syndromic surveillance. We collected wastewater from 63 sewered and non-sewered locations in Bangkok city center between May and August 2022. Monkeypox viral DNA copy numbers were quantified using real-time polymerase chain reaction (PCR) and confirmed positive by Sanger sequencing. Monkeypox viral DNA was first detected in wastewater from the second week of June 2022, with a mean copy number of 16.4 copies/ml (n = 3). From the first week of July, the number of viral DNA copies increased to a mean copy number of 45.92 copies/ml. Positive samples were Sanger sequenced and confirmed the presence of the monkeypox virus. Our study is the first to detect monkeypox viral DNA in wastewater from various locations within Thailand. Results suggest that this could be a complementary source for detecting viral DNA and predicting upcoming outbreaks.",

keywords = "Monkeypox, Monkeypox Asia, Monkeypox viral DNA, Monkeypox virus in wastewater, WBE, Wastewater surveillance",

author = "Wannigama, {Dhammika Leshan} and Mohan Amarasiri and Parichart Hongsing and Cameron Hurst and Charin Modchang and Sudarat Chadsuthi and Suparinthon Anupong and Phatthranit Phattharapornjaroen and {Ali Hosseini}, {Ali Hosseini Rad} and Stefan Fernandez and Huang, {Angkana T.} and Naris Kueakulpattana and Chanikan Tanasatitchai and Porames Vatanaprasan and Thammakorn Saethang and Sirirat Luk-in and Storer, {Robin James} and Puey Ounjai and Ragupathi, {Naveen Kumar Devanga} and Phitsanuruk Kanthawee and Daisuke Sano and Takashi Furukawa and Kazunari Sei and Asada Leelahavanichkul and Talerngsak Kanjanabuch and Nattiya Hirankarn and Higgins, {Paul G.} and Anthony Kicic and Tanittha Chatsuwan and McLellan, {Alexander D.} and Shuichi Abe",

note = "Funding Information: Dhammika Leshan Wannigama was supported by Balvi Filantropic Fund and Chulalongkorn University Second Century Fund-C2F Postdoctoral Fellowship, University of Western Australia (Overseas Research Experience Fellowship) and Yamagata Prefectural Central Hospital, Yamagata, Japan (Clinical Residency Fellowship). Charin Modchang was supported by the Centre of Excellence in Mathematics, Ministry of Higher Education , Science, Research and Innovation, Thailand, Center of Excellence on Medical Biotechnology ( CEMB ), and Thailand Center of Excellence in Physics (ThEP). Anthony Kicic is a Rothwell Family Fellow. Funding Information: Dhammika Leshan Wannigama was supported by Balvi Filantropic Fund and Chulalongkorn University Second Century Fund-C2F Postdoctoral Fellowship, University of Western Australia (Overseas Research Experience Fellowship) and Yamagata Prefectural Central Hospital, Yamagata, Japan (Clinical Residency Fellowship). Charin Modchang was supported by the Centre of Excellence in Mathematics, Ministry of Higher Education, Science, Research and Innovation, Thailand, Center of Excellence on Medical Biotechnology (CEMB), and Thailand Center of Excellence in Physics (ThEP). Anthony Kicic is a Rothwell Family Fellow. Publisher Copyright: {\textcopyright} 2022 Elsevier B.V.",

year = "2023",

month = feb,

day = "1",

doi = "10.1016/j.scitotenv.2022.159816",

language = "English",

volume = "858",

journal = "Science of the Total Environment",

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T1 - Multiple traces of monkeypox detected in non-sewered wastewater with sparse sampling from a densely populated metropolitan area in Asia

AU - Wannigama, Dhammika Leshan

AU - Amarasiri, Mohan

AU - Hongsing, Parichart

AU - Hurst, Cameron

AU - Modchang, Charin

AU - Chadsuthi, Sudarat

AU - Anupong, Suparinthon

AU - Phattharapornjaroen, Phatthranit

AU - Ali Hosseini, Ali Hosseini Rad

AU - Fernandez, Stefan

AU - Huang, Angkana T.

AU - Kueakulpattana, Naris

AU - Tanasatitchai, Chanikan

AU - Vatanaprasan, Porames

AU - Saethang, Thammakorn

AU - Luk-in, Sirirat

AU - Storer, Robin James

AU - Ounjai, Puey

AU - Ragupathi, Naveen Kumar Devanga

AU - Kanthawee, Phitsanuruk

AU - Sano, Daisuke

AU - Furukawa, Takashi

AU - Sei, Kazunari

AU - Leelahavanichkul, Asada

AU - Kanjanabuch, Talerngsak

AU - Hirankarn, Nattiya

AU - Higgins, Paul G.

AU - Kicic, Anthony

AU - Chatsuwan, Tanittha

AU - McLellan, Alexander D.

AU - Abe, Shuichi

N1 - Funding Information: Dhammika Leshan Wannigama was supported by Balvi Filantropic Fund and Chulalongkorn University Second Century Fund-C2F Postdoctoral Fellowship, University of Western Australia (Overseas Research Experience Fellowship) and Yamagata Prefectural Central Hospital, Yamagata, Japan (Clinical Residency Fellowship). Charin Modchang was supported by the Centre of Excellence in Mathematics, Ministry of Higher Education , Science, Research and Innovation, Thailand, Center of Excellence on Medical Biotechnology ( CEMB ), and Thailand Center of Excellence in Physics (ThEP). Anthony Kicic is a Rothwell Family Fellow. Funding Information: Dhammika Leshan Wannigama was supported by Balvi Filantropic Fund and Chulalongkorn University Second Century Fund-C2F Postdoctoral Fellowship, University of Western Australia (Overseas Research Experience Fellowship) and Yamagata Prefectural Central Hospital, Yamagata, Japan (Clinical Residency Fellowship). Charin Modchang was supported by the Centre of Excellence in Mathematics, Ministry of Higher Education, Science, Research and Innovation, Thailand, Center of Excellence on Medical Biotechnology (CEMB), and Thailand Center of Excellence in Physics (ThEP). Anthony Kicic is a Rothwell Family Fellow. Publisher Copyright: © 2022 Elsevier B.V.

PY - 2023/2/1

Y1 - 2023/2/1

N2 - The monkeypox virus is excreted in the feces of infected individuals. Therefore, there is an interest in using viral load detection in wastewater for sentinel early surveillance at a community level and as a complementary approach to syndromic surveillance. We collected wastewater from 63 sewered and non-sewered locations in Bangkok city center between May and August 2022. Monkeypox viral DNA copy numbers were quantified using real-time polymerase chain reaction (PCR) and confirmed positive by Sanger sequencing. Monkeypox viral DNA was first detected in wastewater from the second week of June 2022, with a mean copy number of 16.4 copies/ml (n = 3). From the first week of July, the number of viral DNA copies increased to a mean copy number of 45.92 copies/ml. Positive samples were Sanger sequenced and confirmed the presence of the monkeypox virus. Our study is the first to detect monkeypox viral DNA in wastewater from various locations within Thailand. Results suggest that this could be a complementary source for detecting viral DNA and predicting upcoming outbreaks.

AB - The monkeypox virus is excreted in the feces of infected individuals. Therefore, there is an interest in using viral load detection in wastewater for sentinel early surveillance at a community level and as a complementary approach to syndromic surveillance. We collected wastewater from 63 sewered and non-sewered locations in Bangkok city center between May and August 2022. Monkeypox viral DNA copy numbers were quantified using real-time polymerase chain reaction (PCR) and confirmed positive by Sanger sequencing. Monkeypox viral DNA was first detected in wastewater from the second week of June 2022, with a mean copy number of 16.4 copies/ml (n = 3). From the first week of July, the number of viral DNA copies increased to a mean copy number of 45.92 copies/ml. Positive samples were Sanger sequenced and confirmed the presence of the monkeypox virus. Our study is the first to detect monkeypox viral DNA in wastewater from various locations within Thailand. Results suggest that this could be a complementary source for detecting viral DNA and predicting upcoming outbreaks.

KW - Monkeypox

KW - Monkeypox Asia

KW - Monkeypox viral DNA

KW - Monkeypox virus in wastewater

KW - WBE

KW - Wastewater surveillance

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Multiple traces of monkeypox detected in non-sewered wastewater with sparse sampling from a densely populated metropolitan area in Asia

Abstract

Keywords

ASJC Scopus subject areas

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