Mutational alterations of the key cis proline residue that cause accumulation of enzymatic reaction intermediates of DsbA, a member of the thioredoxin superfamily

Hiroshi Kadokura, Lorenzo Nichols, Jon Beckwith

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

The DsbA-DsbB pathway introduces disulfide bonds into newly translocated proteins. Conversion of the conserved cis proline 151 of DsbA to several hydrophilic residues results in accumulation of mixed disulfides between DsbA and its dedicated oxidant, DsbB. However, only a proline-to-threonine change causes accumulation of mixed disulfides of DsbA with its substrates.

Original languageEnglish
Pages (from-to)1519-1522
Number of pages4
JournalJournal of bacteriology
Volume187
Issue number4
DOIs
Publication statusPublished - 2005 Feb
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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