N-acetyl cysteine alleviates cytotoxicity of bone substitute

M. Yamada, T. Ueno, H. Minamikawa, N. Sato, F. Iwasa, N. Hori, T. Ogawa

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)


Lack of cytocompatibility in bone substitutes impairs healing in surrounding bone. Adverse biological events around biomaterials may be associated with oxidative stress. We hypothesized that a clinically used inorganic bone substitute is cytotoxic to osteoblasts due to oxidative stress and that N-acetyl cysteine (NAC), an antioxidant amino acid derivative, would detoxify such material. Only 20% of rat calvaria osteoblasts were viable when cultured on commercial deproteinized bovine bone particles for 24 hr, whereas this percentage doubled on bone substitute containing NAC. Intracellular ROS levels markedly increased on and under bone substitutes, which were reduced by prior addition of NAC to materials. NAC restored suppressed alkaline phosphatase activity in the bone substitute. Proinflammatory cytokine levels from human osteoblasts on the bone substitute decreased by one-third or more with addition of NAC. NAC alleviated cytotoxicity of the bone substitute to osteoblastic viability and function, implying enhanced bone regeneration around NAC-treated inorganic biomaterials.

Original languageEnglish
Pages (from-to)411-416
Number of pages6
JournalJournal of Dental Research
Issue number4
Publication statusPublished - 2010 Apr


  • Anti-oxidant
  • Apoptosis
  • Bone regeneration
  • Inorganic biomaterial.
  • Reactive oxygen species (ROS)


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