NAD+-glycohydrolase from Streptococcus pyogenes shows cyclic ADP-ribose forming activity

Tadahiro Karasawa; Shin Takasawa; Kiyotaka Yamakawa; Hideto Yonekura; Hiroshi Okamoto; Shinichi Nakamura

doi:10.1016/0378-1097(95)00206-K

NAD⁺-glycohydrolase from Streptococcus pyogenes shows cyclic ADP-ribose forming activity

Tadahiro Karasawa, Shin Takasawa, Kiyotaka Yamakawa, Hideto Yonekura, Hiroshi Okamoto, Shinichi Nakamura

Surgery

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

NAD⁺-glycohydrolase from Streptococcus pyogenes was purified by successive chromatography on CM Sepharose CL-6B, Sephacryl S-200 HR and hydroxyapatite. The purified enzyme possessed synthesis and hydrolysis activities of cyclic ADP-ribose (cADPR), a newly found second messenger for Ca²⁺ mobilisation, along with cleavage activity of the ribose-nicotinamide bond in NAD⁺.

Original language	English
Pages (from-to)	201-204
Number of pages	4
Journal	FEMS Microbiology Letters
Volume	130
Issue number	2-3
DOIs	https://doi.org/10.1016/0378-1097(95)00206-K
Publication status	Published - 1995 Aug 1

Keywords

ADP-ribosyl cyclase
Cyclic ADP-ribose
Cyclic ADP-ribose hydrolase
NAD-glycohydrolase
Streptococcus pyogenes

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

Access to Document

10.1016/0378-1097(95)00206-K

Cite this

@article{dfe2f4e0e2e7474e91b4d10e95a34f5c,

title = "NAD+-glycohydrolase from Streptococcus pyogenes shows cyclic ADP-ribose forming activity",

abstract = "NAD+-glycohydrolase from Streptococcus pyogenes was purified by successive chromatography on CM Sepharose CL-6B, Sephacryl S-200 HR and hydroxyapatite. The purified enzyme possessed synthesis and hydrolysis activities of cyclic ADP-ribose (cADPR), a newly found second messenger for Ca2+ mobilisation, along with cleavage activity of the ribose-nicotinamide bond in NAD+.",

keywords = "ADP-ribosyl cyclase, Cyclic ADP-ribose, Cyclic ADP-ribose hydrolase, NAD-glycohydrolase, Streptococcus pyogenes",

author = "Tadahiro Karasawa and Shin Takasawa and Kiyotaka Yamakawa and Hideto Yonekura and Hiroshi Okamoto and Shinichi Nakamura",

year = "1995",

month = aug,

day = "1",

doi = "10.1016/0378-1097(95)00206-K",

language = "English",

volume = "130",

pages = "201--204",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Wiley-Blackwell",

number = "2-3",

}

TY - JOUR

T1 - NAD+-glycohydrolase from Streptococcus pyogenes shows cyclic ADP-ribose forming activity

AU - Karasawa, Tadahiro

AU - Takasawa, Shin

AU - Yamakawa, Kiyotaka

AU - Yonekura, Hideto

AU - Okamoto, Hiroshi

AU - Nakamura, Shinichi

PY - 1995/8/1

Y1 - 1995/8/1

N2 - NAD+-glycohydrolase from Streptococcus pyogenes was purified by successive chromatography on CM Sepharose CL-6B, Sephacryl S-200 HR and hydroxyapatite. The purified enzyme possessed synthesis and hydrolysis activities of cyclic ADP-ribose (cADPR), a newly found second messenger for Ca2+ mobilisation, along with cleavage activity of the ribose-nicotinamide bond in NAD+.

AB - NAD+-glycohydrolase from Streptococcus pyogenes was purified by successive chromatography on CM Sepharose CL-6B, Sephacryl S-200 HR and hydroxyapatite. The purified enzyme possessed synthesis and hydrolysis activities of cyclic ADP-ribose (cADPR), a newly found second messenger for Ca2+ mobilisation, along with cleavage activity of the ribose-nicotinamide bond in NAD+.

KW - ADP-ribosyl cyclase

KW - Cyclic ADP-ribose

KW - Cyclic ADP-ribose hydrolase

KW - NAD-glycohydrolase

KW - Streptococcus pyogenes

UR - http://www.scopus.com/inward/record.url?scp=0029130463&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029130463&partnerID=8YFLogxK

U2 - 10.1016/0378-1097(95)00206-K

DO - 10.1016/0378-1097(95)00206-K

M3 - Article

C2 - 7649441

AN - SCOPUS:0029130463

SN - 0378-1097

VL - 130

SP - 201

EP - 204

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2-3

ER -