TY - JOUR
T1 - Nano-manipulation of actomyosin molecular motors in vitro
T2 - a new working principle
AU - Yanagida, Toshio
AU - Harada, Yoshie
AU - Ishijima, Akihiko
PY - 1993/9
Y1 - 1993/9
N2 - Techniques have been recently developed that allow the direct observation of single actin filaments and their manipulation, using glass microneedles, in the nanometer range. Further development of these techniques has made possible the detection of subpiconewton-level forces of individual myosin heads. This in vitro motility model is sensitive in the submillisecond range and has allowed us to determine the force generation of an actomyosin motor directly at the molecular level. The results have led to a new conceptual framework for chemo-mechanical energy transduction in the molecular motor.
AB - Techniques have been recently developed that allow the direct observation of single actin filaments and their manipulation, using glass microneedles, in the nanometer range. Further development of these techniques has made possible the detection of subpiconewton-level forces of individual myosin heads. This in vitro motility model is sensitive in the submillisecond range and has allowed us to determine the force generation of an actomyosin motor directly at the molecular level. The results have led to a new conceptual framework for chemo-mechanical energy transduction in the molecular motor.
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U2 - 10.1016/0968-0004(93)90064-T
DO - 10.1016/0968-0004(93)90064-T
M3 - Article
C2 - 8236451
AN - SCOPUS:0027296257
SN - 0376-5067
VL - 18
SP - 319
EP - 324
JO - Trends in Biochemical Sciences
JF - Trends in Biochemical Sciences
IS - 9
ER -