@article{1ff77221ea5141279d05d181db187708,
title = "Nanosecond pump-probe device for time-resolved serial femtosecond crystallography developed at SACLA",
abstract = "X-ray free-electron lasers (XFELs) have opened new opportunities for time-resolved X-ray crystallography. Here a nanosecond optical-pump XFEL-probe device developed for time-resolved serial femtosecond crystallography (TR-SFX) studies of photo-induced reactions in proteins at the SPring-8 Angstrom Compact free-electron LAser (SACLA) is reported. The optical-fiber-based system is a good choice for a quick setup in a limited beam time and allows pump illumination from two directions to achieve high excitation efficiency of protein microcrystals. Two types of injectors are used: one for extruding highly viscous samples such as lipidic cubic phase (LCP) and the other for pulsed liquid droplets. Under standard sample flow conditions from the viscous-sample injector, delay times from nanoseconds to tens of milliseconds are accessible, typical time scales required to study large protein conformational changes. A first demonstration of a TR-SFX experiment on bacteriorhodopsin in bicelle using a setup with a droplet-type injector is also presented.A nanosecond pump-probe device for time-resolved serial femtosecond crystallography has been developed at SACLA.",
keywords = "XFEL, pump and probe, serial femtosecond crystallography, time-resolved X-ray crystallography",
author = "Minoru Kubo and Eriko Nango and Kensuke Tono and Tetsunari Kimura and Shigeki Owada and Changyong Song and Fumitaka Mafun{\'e} and Ken Miyajima and Yoshihiro Takeda and Kohno, {Jun Ya} and Naoya Miyauchi and Takanori Nakane and Tomoyuki Tanaka and Takashi Nomura and Jan Davidsson and Rie Tanaka and Michio Murata and Takashi Kameshima and Takaki Hatsui and Yasumasa Joti and Richard Neutze and Makina Yabashi and So Iwata",
note = "Funding Information: We thank Dr E. Mizohata, Dr M. Sugahara, Dr M. Suzuki, Dr T. Masuda, Dr T. Hosaka, Dr H. Naitow, Mr Y. Matsuura and Ms A. Yamashita for data collection, Ms T. Arima for crystallization, Dr Shigeru Matsuoka and Dr Yuichi Umekawa for preparation of the purple membrane, and members of the Engineering Team of RIKEN SPring-8 Center, especially Mr Y. Shimazu, Mr K. Hata, Mr N. Suzuki and Mr T. Kin, for their technical support. XFEL experiments were conducted at BL3 of SACLA with the approval of the Japan Synchrotron Radiation Research Institute (JASRI) (proposal numbers 2016B8063 and 2017A8018). We acknowledge computational support from SACLA HPC system and Mini-K super computer system. MK acknowledges financial support from a JSPS Grant-in-Aid for Scientific Research on Innovative Areas {\textquoteleft}3D Active-Site Science{\textquoteright} (15H01055) and Scientific Research (B) (15H03841). JD acknowledges support from the Swedish Research Council (VR). RN acknowledges financial support from the Swedish Research Council (VR), the Swedish Foundation for Strategic Research (SSF) and the Knut and Alice Wallenberg Foundation (KAW). CS is supported by National Research Foundation (see grant details below). Funding Information: The following funding is acknowledged: X-ray Free-Electron Laser Priority Strategy Program (MEXT); Strategic Basic Research Program (JST); Pioneering Project {"}Dynamic Structural Biology{"} of RIKEN; Vetenskapsr{\aa}det; Stiftelsen f{\"o}r Strategisk Forskning; Knut och Alice Wallenbergs Stif-telse; National Research Foundation of Korea (award No. 2015R1A5A1009962; award No. 2016R1A2B3010980). Publisher Copyright: {\textcopyright} Minoru Kubo et al. 2017.",
year = "2017",
month = sep,
doi = "10.1107/S160057751701030X",
language = "English",
volume = "24",
pages = "1086--1091",
journal = "Journal of Synchrotron Radiation",
issn = "0909-0495",
publisher = "International Union of Crystallography",
number = "5",
}