Nanosecond pump-probe device for time-resolved serial femtosecond crystallography developed at SACLA

Minoru Kubo, Eriko Nango, Kensuke Tono, Tetsunari Kimura, Shigeki Owada, Changyong Song, Fumitaka Mafuné, Ken Miyajima, Yoshihiro Takeda, Jun Ya Kohno, Naoya Miyauchi, Takanori Nakane, Tomoyuki Tanaka, Takashi Nomura, Jan Davidsson, Rie Tanaka, Michio Murata, Takashi Kameshima, Takaki Hatsui, Yasumasa JotiRichard Neutze, Makina Yabashi, So Iwata

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


X-ray free-electron lasers (XFELs) have opened new opportunities for time-resolved X-ray crystallography. Here a nanosecond optical-pump XFEL-probe device developed for time-resolved serial femtosecond crystallography (TR-SFX) studies of photo-induced reactions in proteins at the SPring-8 Angstrom Compact free-electron LAser (SACLA) is reported. The optical-fiber-based system is a good choice for a quick setup in a limited beam time and allows pump illumination from two directions to achieve high excitation efficiency of protein microcrystals. Two types of injectors are used: one for extruding highly viscous samples such as lipidic cubic phase (LCP) and the other for pulsed liquid droplets. Under standard sample flow conditions from the viscous-sample injector, delay times from nanoseconds to tens of milliseconds are accessible, typical time scales required to study large protein conformational changes. A first demonstration of a TR-SFX experiment on bacteriorhodopsin in bicelle using a setup with a droplet-type injector is also presented.A nanosecond pump-probe device for time-resolved serial femtosecond crystallography has been developed at SACLA.

Original languageEnglish
Pages (from-to)1086-1091
Number of pages6
JournalJournal of Synchrotron Radiation
Issue number5
Publication statusPublished - 2017 Sept


  • XFEL
  • pump and probe
  • serial femtosecond crystallography
  • time-resolved X-ray crystallography


Dive into the research topics of 'Nanosecond pump-probe device for time-resolved serial femtosecond crystallography developed at SACLA'. Together they form a unique fingerprint.

Cite this