Neutron scattering study of phosphatidylinositol 4,5-bis(phosphate) aqueous dispersion

M. Hirai; T. Takizawa; K. Hayashi; S. Yabuki; M. Imai; Y. Matsushita; Y. Ito

doi:10.1007/BF01189527

Neutron scattering study of phosphatidylinositol 4,5-bis(phosphate) aqueous dispersion

M. Hirai, T. Takizawa, K. Hayashi, S. Yabuki, M. Imai, Y. Matsushita, Y. Ito

Research output: Contribution to journal › Article › peer-review

Abstract

In this paper we treat the thermotropic phase behavior of phosphatidylinositol 4,5-bis(phosphate) (PIP₂) aggregates in aqueous solution. By using the solvent contrast variation method in neutron small-angle scattering we could determine the absolute value of the PIP₂ molecular volume in the micellar form from the contrast matching point. The shift of the contrast matching point indicates the increase of the PIP₂ molecular volume of ∼11% with elevating temperature from 10°C to 50°C. The decrease of the gyration radius of the PIP₂ micelle observed in the present experiments well agrees with our previous results of X-ray scattering study. The present results suggest that the increase of the molecular volume would be mainly attributable to the change of the hydrophilic polar head region to decrease the critical packing parameter.

Original language	English
Pages (from-to)	232-236
Number of pages	5
Journal	Progress in Colloid and Polymer Science
Volume	106
DOIs	https://doi.org/10.1007/BF01189527
Publication status	Published - 1997 Jan 1
Externally published	Yes

Keywords

Contrast variation
Neutron scattering
Phosphatidylinositol
Small-angle scattering
Thermal transition

ASJC Scopus subject areas

Physical and Theoretical Chemistry
Organic Chemistry
Polymers and Plastics
Colloid and Surface Chemistry
Materials Chemistry

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10.1007/BF01189527

Cite this

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title = "Neutron scattering study of phosphatidylinositol 4,5-bis(phosphate) aqueous dispersion",

abstract = "In this paper we treat the thermotropic phase behavior of phosphatidylinositol 4,5-bis(phosphate) (PIP2) aggregates in aqueous solution. By using the solvent contrast variation method in neutron small-angle scattering we could determine the absolute value of the PIP2 molecular volume in the micellar form from the contrast matching point. The shift of the contrast matching point indicates the increase of the PIP2 molecular volume of ∼11% with elevating temperature from 10°C to 50°C. The decrease of the gyration radius of the PIP2 micelle observed in the present experiments well agrees with our previous results of X-ray scattering study. The present results suggest that the increase of the molecular volume would be mainly attributable to the change of the hydrophilic polar head region to decrease the critical packing parameter.",

keywords = "Contrast variation, Neutron scattering, Phosphatidylinositol, Small-angle scattering, Thermal transition",

author = "M. Hirai and T. Takizawa and K. Hayashi and S. Yabuki and M. Imai and Y. Matsushita and Y. Ito",

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T1 - Neutron scattering study of phosphatidylinositol 4,5-bis(phosphate) aqueous dispersion

AU - Hirai, M.

AU - Takizawa, T.

AU - Hayashi, K.

AU - Yabuki, S.

AU - Imai, M.

AU - Matsushita, Y.

AU - Ito, Y.

PY - 1997/1/1

Y1 - 1997/1/1

N2 - In this paper we treat the thermotropic phase behavior of phosphatidylinositol 4,5-bis(phosphate) (PIP2) aggregates in aqueous solution. By using the solvent contrast variation method in neutron small-angle scattering we could determine the absolute value of the PIP2 molecular volume in the micellar form from the contrast matching point. The shift of the contrast matching point indicates the increase of the PIP2 molecular volume of ∼11% with elevating temperature from 10°C to 50°C. The decrease of the gyration radius of the PIP2 micelle observed in the present experiments well agrees with our previous results of X-ray scattering study. The present results suggest that the increase of the molecular volume would be mainly attributable to the change of the hydrophilic polar head region to decrease the critical packing parameter.

AB - In this paper we treat the thermotropic phase behavior of phosphatidylinositol 4,5-bis(phosphate) (PIP2) aggregates in aqueous solution. By using the solvent contrast variation method in neutron small-angle scattering we could determine the absolute value of the PIP2 molecular volume in the micellar form from the contrast matching point. The shift of the contrast matching point indicates the increase of the PIP2 molecular volume of ∼11% with elevating temperature from 10°C to 50°C. The decrease of the gyration radius of the PIP2 micelle observed in the present experiments well agrees with our previous results of X-ray scattering study. The present results suggest that the increase of the molecular volume would be mainly attributable to the change of the hydrophilic polar head region to decrease the critical packing parameter.

KW - Contrast variation

KW - Neutron scattering

KW - Phosphatidylinositol

KW - Small-angle scattering

KW - Thermal transition

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Neutron scattering study of phosphatidylinositol 4,5-bis(phosphate) aqueous dispersion

Abstract

Keywords

ASJC Scopus subject areas

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