TY - JOUR
T1 - Neutrophil chemoattractant 2β regulates expression of the Reg gene in injured gastric mucosa in rats
AU - Kazumori, Hideaki
AU - Ishihara, Shunji
AU - Hoshino, Eiichi
AU - Kawashima, Kousaku
AU - Moriyama, Nobuyuki
AU - Suetsugu, Hiroshi
AU - Sato, Hiroshi
AU - Adachi, Kyoichi
AU - Fukuda, Ryo
AU - Watanabe, Makoto
AU - Takasawa, Shin
AU - Okamoto, Hiroshi
AU - Fukui, Hirokazu
AU - Chiba, Tsutomu
AU - Kinoshita, Yoshikazu
N1 - Funding Information:
Supported in part by grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture of Japan.
PY - 2000
Y1 - 2000
N2 - Background & Aims: Regenerating (Reg) protein has a trophic effect on gastric mucosal cells. We have shown that Reg gene expression is increased in enterochromaffin-like (ECL) cells during the healing of damaged gastric mucosa around mucosal erosion. This study was designed to explore the stimulants of Reg expression during the healing of gastric mucosal damage. Methods: Time course changes of the expression of genes for various proinflammatory cytokines and Reg were investigated after induction of gastric mucosal lesions in rats. The direct effect of proinflammatory cytokines on Reg gene expression and Reg protein production were investigated in vitro using counterflow elutriation-enriched rat ECL cells. CXC receptor 2 (CXCR-2) expression was investigated in ECL cells by reverse-transcription polymerase chain reaction. Reg gene expression was also investigated in rats treated by the neutralizing antibody of cytokine-induced neutrophil chemoattractant (CINC-2β). Results: During healing, the gene expression of several proinflammatory cytokines and Reg was markedly augmented. Among the proinflammatory cytokines, CINC-2β is the only cytokine in which augmented expression preceded the increase of Reg gene expression. In rats treated with CINC-2β neutralizing antibody, the augmentation of Reg gene expression was significantly inhibited. When ECL cells were incubated with these proinflammatory cytokines, CINC-2β dose-dependently increased Reg messenger RNA and Reg protein in ECL cells. CXCR-2 was identified in isolated ECL cells. Conclusions: CINC-2β, expressed in damaged gastric mucosa, stimulates the production of Reg protein in ECL cells via CXCR-2 and may be involved in the accelerated healing of injured gastric mucosa.
AB - Background & Aims: Regenerating (Reg) protein has a trophic effect on gastric mucosal cells. We have shown that Reg gene expression is increased in enterochromaffin-like (ECL) cells during the healing of damaged gastric mucosa around mucosal erosion. This study was designed to explore the stimulants of Reg expression during the healing of gastric mucosal damage. Methods: Time course changes of the expression of genes for various proinflammatory cytokines and Reg were investigated after induction of gastric mucosal lesions in rats. The direct effect of proinflammatory cytokines on Reg gene expression and Reg protein production were investigated in vitro using counterflow elutriation-enriched rat ECL cells. CXC receptor 2 (CXCR-2) expression was investigated in ECL cells by reverse-transcription polymerase chain reaction. Reg gene expression was also investigated in rats treated by the neutralizing antibody of cytokine-induced neutrophil chemoattractant (CINC-2β). Results: During healing, the gene expression of several proinflammatory cytokines and Reg was markedly augmented. Among the proinflammatory cytokines, CINC-2β is the only cytokine in which augmented expression preceded the increase of Reg gene expression. In rats treated with CINC-2β neutralizing antibody, the augmentation of Reg gene expression was significantly inhibited. When ECL cells were incubated with these proinflammatory cytokines, CINC-2β dose-dependently increased Reg messenger RNA and Reg protein in ECL cells. CXCR-2 was identified in isolated ECL cells. Conclusions: CINC-2β, expressed in damaged gastric mucosa, stimulates the production of Reg protein in ECL cells via CXCR-2 and may be involved in the accelerated healing of injured gastric mucosa.
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U2 - 10.1053/gast.2000.20262
DO - 10.1053/gast.2000.20262
M3 - Article
C2 - 11113082
AN - SCOPUS:0034464250
SN - 0016-5085
VL - 119
SP - 1610
EP - 1622
JO - Gastroenterology
JF - Gastroenterology
IS - 6
ER -